OsWRKY80-OsWRKY4 Module as a Positive Regulatory Circuit in Rice Resistance Against Rhizoctonia solani.
OsWRKY80-OsWRKY4 Module as a Positive Regulatory Circuit in Rice Resistance Against Rhizoctonia solani.
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OsWRKY80-OsWRKY4 模块作为水稻抗立枯丝核菌的正调节电路
DOI：
10.1186/s12284-016-0137-y
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Tang X
中科院分区：
文献类型：
--
作者：
Peng X;Wang H;Jang JC;Xiao T;He H;Jiang D;Tang X
背景：植物WRKY转录因子在多种生物学过程中发挥关键作用，但最显著的是在植物对病原体的防御反应中发挥作用。纹枯病是水稻的主要病害之一。结果：水稻纹枯病病原菌纹枯病菌侵染水稻纹枯病后，WRKY80基因(OsWRKY80)迅速而强烈地诱导表达。外源茉莉酸(JA)和乙烯(ET)也能诱导OsWRKY80的表达，但水杨酸(SA)不能诱导OsWRKY80的表达。在瞬时表达实验中，OsWRKY80-GFP定位于洋葱表皮细胞的细胞核中。在酵母细胞的GAL4实验中，OsWRKY80始终显示出转录激活活性。在水稻植株中过表达OsWRKY80显著提高了水稻对纹枯病菌的抗病能力，同时水稻对纹枯病菌的另一种正向调控基因OsWRKY4的表达也增加了。另一方面，通过RNA干扰(RNAi)抑制OsWRKY80，降低了对纹枯病菌的抗病能力。酵母单杂交和烟草细胞瞬时表达分析结果表明，OsWRKY80与OsWRKY4的启动子区域特异结合，含有W-box(TTGAC[C/T])或W-box like(TGAC[C/T])顺式元件。结论：OsWRKY80在OsWRKY4的上游起重要的正向调控作用，参与水稻对纹枯病病菌的防御反应。
Background:Plant WRKY transcription factors play pivotal roles in diverse biological processes but most notably in plant defense response to pathogens. Sheath blight represents one of the predominant diseases in rice. However, our knowledge about the functions of WRKY proteins in rice defense against sheath blight is rather limited.Results:Here we demonstrate that the expression of Oryza sativa WRKY80 gene (OsWRKY80) is rapidly and strongly induced upon infection of Rhizoctonia solani, the causal agent of rice sheath blight disease. OsWRKY80 expression is also induced by exogenous jasmonic acid (JA) and ethylene (ET), but not by salicylic acid (SA). OsWRKY80-GFP is localized in the nuclei of onion epidermal cells in a transient expression assay. Consistently, OsWRKY80 exhibits transcriptional activation activity in a GAL4 assay in yeast cells. Overexpression of OsWRKY80 in rice plants significantly enhanced disease resistance to R. solani, concomitant with elevated expression of OsWRKY4, another positive regulator in rice defense against R. solani. Suppression of OsWRKY80 by RNA interference (RNAi), on the other hand, compromised disease resistance to R. solani. Results of yeast one-hybrid assay and transient expression assay in tobacco cells have revealed that OsWRKY80 specifically binds to the promoter regions of OsWRKY4, which contain W-box (TTGAC[C/T]) or W-box like (TGAC[C/T]) cis-elements.Conclusions:We propose that OsWRKY80 functions upstream of OsWRKY4 as an important positive regulatory circuit that is implicated in rice defense response to sheath blight pathogen R. solani.
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影响因子：
4.9
作者：
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