Spoligotyping and drug resistance analysis of Mycobacterium tuberculosis strains from national survey in China.
Spoligotyping and drug resistance analysis of Mycobacterium tuberculosis strains from national survey in China.
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DOI：
10.1371/journal.pone.0032976
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhao YL
中科院分区：
文献类型：
--
作者：
Pang Y;Zhou Y;Zhao B;Liu G;Jiang G;Xia H;Song Y;Shang Y;Wang S;Zhao YL
由结核分枝杆菌复合群（MTBC）引起的结核病（TB）是当今世界上主要的死亡原因之一。虽然中国是全球结核病发病率第二大的国家，但对中国结核病患病率的系统研究尚未完成。2006 - 2007年，卫生部开展了结核病基线监测，从全国31个省、自治区、直辖市共筛选出4000余株代表菌株。本研究的目的是调查代表性结核分枝杆菌（M.应用间隔区寡核苷酸分型技术（spoligotyping）对中国结核分枝杆菌进行基因分型，并分析基因型与耐药性的关系。从2007年至2008年，在中国各地共收集到4017株临床分离株。其中M。结核病分离株中，北京基因型2500株（62.2%）。北京基因型在北方的比例高于南方（76.5%比53.2%）。北京基因型菌株对利福平、氧氟沙星和多重耐药的频率明显高于非北京基因型菌株。此外，一种新的基因型命名为“中国南方基因型（CS）”，只从福建和广东省。因此，揭示CS型在中国南方流行的原因是非常实际的。结论：北京家族基因型仍是我国结核分枝杆菌的优势基因型，与利福平耐药、氧氟沙星耐药和MDR表型的相关性较其他寡核苷酸基因型高，且我国部分地区结核分枝杆菌的分布具有独特性。结核病基因型我们的研究为结核病控制和研究界做出了重要贡献，完成了关于结核分枝杆菌耐药性水平和分布的广泛研究。结核菌株类型。
Tuberculosis (TB), caused by Mycobacterium tuberculosis complex (MTBC), is one of the major causes of death in the world today. Although China has the second largest global case rate of tuberculosis, a systematic study of TB prevalence in China has not been completed. From 2006 to 2007, the base line surveillance of tuberculosis was carried out by Ministry of Health, and more than 4000 representative strains were selected from 31 provinces in China. The aim of the present research was to survey the genotypes of representative Mycobacterium tuberculosis (M. tuberculosis) strains from China using spacer oligonucleotide typing (spoligotyping), and to analyze the relationship between genotype and drug resistance for the first time. A total of 4017 clinical isolates were collected from 2007 to 2008 throughout China. Among those M. tuberculosis isolates, 2500 (62.2%) isolates were Beijing genotypes. The percentage of Beijing genotypes in northern China was higher than in southern China (76.5% vs. 53.2%). Additionally, the frequencies of rifampin-resistant, ofloxacin-resistant and multidrug-resistant isolates were significantly higher in Beijing genotype strains than non-Beijing strains. Furthermore, a novel genotype named “China Southern genotype (CS)” was only isolated from Fujian and Guangdong provinces. Hence, it is very practical to uncover the reason for prevalence of the CS type in southern China. In conclusion, Beijing family genotypes were still the predominant genotype throughout China, which exhibited a greater correlation with rifampin-resistance, ofloxacin-resistance and MDR phenotypes than other TB spoligotypes, and some regions of China showed several unique characters in the distribution of M. tuberculosis genotypes. Our research represents an important contribution for the TB control and research community, which completes broad pictures on drug resistance levels and distribution of M. tuberculosis strain types over China.
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