TGF-β1 signaling is essential for tissue regeneration in the Xenopus tadpole tail.
TGF-β1 signaling is essential for tissue regeneration in the Xenopus tadpole tail.
复制标题
DOI：
10.1016/j.bbrc.2021.05.082
复制
发表时间：
2021-08-06
影响因子：
3.1
通讯作者：
Suzuki A
中科院分区：
文献类型：
--
作者：
Nakamura M;Yoshida H;Moriyama Y;Kawakita I;Wlizla M;Takebayashi-Suzuki K;Horb ME;Suzuki A
两栖动物如热带爪蟾在创伤后表现出显著的组织再生能力。虽然已知转化生长因子-β（TGF-β）受体信号传导对于鱼类和两栖动物的组织再生是必不可少的，但TGF-β配体在该过程中的作用还不清楚。在这里，我们表明，抑制TGF-β1功能，防止尾巴再生爪蟾tropicalis蝌蚪。我们发现tgfb 1的表达在断尾前就存在，并在整个再生过程中持续存在。CRISPR介导的tgfb 1敲除（KO）延迟了尾部再生; tgfb 1 KO蝌蚪的表型可以通过注射tgfb 1 mRNA来拯救。细胞增殖是组织再生成功的关键事件，在tgfb 1 KO蝌蚪中下调。此外，tgfb 1 KO降低磷酸化Smad 2/3（pSmad 2/3）的表达，这对于TGF-β信号介导的细胞增殖是重要的。总的来说，我们的研究结果表明，TGF-β1通过激活Smad 2/3调节细胞增殖。因此，我们认为TGF-β1在非洲爪蟾依赖TGF-β受体的蝌蚪尾部再生中起着关键作用。
Amphibians such as Xenopus tropicalis exhibit a remarkable capacity for tissue regeneration after traumatic injury. Although transforming growth factor-β (TGF-β) receptor signaling is known to be essential for tissue regeneration in fish and amphibians, the role of TGF-β ligands in this process is not well understood. Here, we show that inhibition of TGF-β1 function prevents tail regeneration in Xenopus tropicalis tadpoles. We found that expression of tgfb1 is present before tail amputation and is sustained throughout the regeneration process. CRISPR-mediated knock-out (KO) of tgfb1 retards tail regeneration; the phenotype of tgfb1 KO tadpoles can be rescued by injection of tgfb1 mRNA. Cell proliferation, a critical event for the success of tissue regeneration, is downregulated in tgfb1 KO tadpoles. In addition, tgfb1 KO reduces the expression of phosphorylated Smad2/3 (pSmad2/3) which is important for TGF-β signal-mediated cell proliferation. Collectively, our results show that TGF-β1 regulates cell proliferation through the activation of Smad2/3. We therefore propose that TGF-β1 plays a critical role in TGF-β receptor-dependent tadpole tail regeneration in Xenopus.
登录
查看更多内容
影响因子：
--
作者：
Nakayama, Takuya;Blitz, Ira L.;Fish, Margaret B.;Odeleye, Akinleye O.;Manohar, Sumanth;Cho, Ken W. Y.;Grainger, Robert M.
通讯作者：
Grainger, Robert M.
影响因子：
3.6
作者：
Byfield, SD;Major, C;Roberts, AB
通讯作者：
Roberts, AB
DOI：
10.1073/pnas.1408233111
发表时间：
2014-06-17
影响因子：
11.1
作者：
Ali, Shah R.;Hippenmeyer, Simon;Ardehali, Reza
通讯作者：
Ardehali, Reza
影响因子：
8
作者：
Mohammadi, Mona Malek;Abouissa, Aya;Heineke, Joerg
通讯作者：
Heineke, Joerg
影响因子：
--
作者：
Taniguchi Y;Watanabe K;Mochii M
通讯作者：
Mochii M