Remote Activation of a Nanopore for High-Performance Genetic Detection Using a pH Taxis-Mimicking Mechanism.
Remote Activation of a Nanopore for High-Performance Genetic Detection Using a pH Taxis-Mimicking Mechanism.
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DOI：
10.1021/acs.analchem.7b03979
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发表时间：
2017-12-19
影响因子：
7.4
通讯作者：
Gu LQ
中科院分区：
文献类型：
--
作者：
Wang Y;Tian K;Du X;Shi RC;Gu LQ
气溶蛋白孔已广泛应用于多肽和蛋白质的传感。然而，只有少数小组探索了这种纳米孔用于核酸检测。挑战在于对核酸的捕获效率极低（10个碱基），这严重降低了基于气溶素的遗传生物传感器的灵敏度。在这里，我们报道了一种简单且易于操作的方法，将气溶素非共价转化为高度核酸敏感的纳米孔。通过远程pH调节机制，我们只需降低孔一侧的pH值，然后气溶素立即被“激活”，并使其能够从孔的另一侧有效地捕获目标DNA/RNA。这种机制也减缓了DNA易位，这是测序和基因检测所需的特性，允许不同长度的DNA在时间上分离。该方法为生物传感的纳米孔工程提供了见解，使气溶素适用于长核酸的遗传和表观遗传检测。
Aerolysin protein pore has been widely used for sensing peptides and proteins. However, only a few groups explored this nanopore for nucleic acids detection. The challenge is the extremely low capture efficiency for nucleic acids (>10 bases), which severely lowers the sensitivity of an aerolysin-based genetic biosensor. Here we reported a simple and easy-to-operate approach to noncovalently transform aerolysin into a highly nucleic acids-sensitive nanopore. Through a remote pH-modulation mechanism, we simply lower the pH on one side of the pore, then aerolysin is immediately “activated” and enabled to capture target DNA/RNA efficiently from the opposite side of the pore. This mechanism also decelerates DNA translocation, a desired property for sequencing and gene detection, allowing temporal separation of DNAs in different lengths. This method provides insight into the nanopore engineering for biosensing, making aerolysin applicable in genetic and epigenetic detections of long nucleic acids.
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影响因子：
14.8
作者：
Degiacomi, Matteo T.;Lacovache, Ioan;Dal Peraro, Matteo
通讯作者：
Dal Peraro, Matteo
DOI：
10.1073/pnas.2531778100
发表时间：
2003-12-23
影响因子：
11.1
作者：
Gu, LQ;Cheley, S;Bayley, H
通讯作者：
Bayley, H
DOI：
10.1073/pnas.1001831107
发表时间：
2010-09-14
影响因子：
11.1
作者：
Derrington, Ian M.;Butler, Tom Z.;Gundlach, Jens H.
通讯作者：
Gundlach, Jens H.
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
38.3
作者：
Cao, Chan;Ying, Yi-Lun;Long, Yi-Tao
通讯作者：
Long, Yi-Tao