A novel nucleoid protein of Escherichia coli induced under anaerobiotic growth conditions.
A novel nucleoid protein of Escherichia coli induced under anaerobiotic growth conditions.
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DOI：
10.1093/nar/gkq077
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发表时间：
2010-06
影响因子：
14.9
通讯作者：
Ishihama A
中科院分区：
文献类型：
--
作者：
Teramoto J;Yoshimura SH;Takeyasu K;Ishihama A
利用基因组SELEX系统搜索大肠杆菌未鉴定的转录因子YgiP的dna结合序列。利用高密度微阵列（SELEX芯片）对SELEX片段进行全基因组分析后，共鉴定出688个ygip结合位点。凝胶移位和DNA - i足迹分析表明，YgiP与DNA探针上的多个位点结合，具有一致的GTTNATT序列。原子力显微镜观察表明，在低浓度下，YgiP在DNA探针上的各个位点结合，但在高浓度下，YgiP可能通过蛋白质-蛋白质接触覆盖整个DNA表面。在有氧条件下生长的大肠杆菌细胞中，YgiP的细胞内浓度很低，但在厌氧条件下增加100倍以上，达到与主要类核蛋白一样高的水平。缺乏ygiP的大肠杆菌突变体在厌氧条件下表现出生长迟缓。高丰度和大量的结合位点共同表明YgiP是一种核相关蛋白，与类核蛋白Fis和IHF一样具有结构和调节作用。然后我们提出YgiP是一种新的厌氧条件下的大肠杆菌类核蛋白，并建议将其重新命名为Dan（厌氧条件下的dna结合蛋白）。
A systematic search was performed for DNA-binding sequences of YgiP, an uncharacterized transcription factor of Escherichia coli, by using the Genomic SELEX. A total of 688 YgiP-binding loci were identified after genome-wide profiling of SELEX fragments with a high-density microarray (SELEX-chip). Gel shift and DNase-I footprinting assays indicated that YgiP binds to multiple sites along DNA probes with a consensus GTTNATT sequence. Atomic force microscope observation indicated that at low concentrations, YgiP associates at various sites on DNA probes, but at high concentrations, YgiP covers the entire DNA surface supposedly through protein–protein contact. The intracellular concentration of YgiP is very low in growing E. coli cells under aerobic conditions, but increases more than 100-fold to the level as high as the major nucleoid proteins under anaerobic conditions. An E. coli mutant lacking ygiP showed retarded growth under anaerobic conditions. High abundance and large number of binding sites together indicate that YgiP is a nucleoid-associated protein with both architectural and regulatory roles as the nucleoid proteins Fis and IHF. We then propose that YgiP is a novel nucleoid protein of E. coli under anaerobiosis and propose to rename it Dan (DNA-binding protein under anaerobic conditions).
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影响因子：
3.2
作者：
Ishida, Yuji;Kori, Ayako;Ishihama, Akira
通讯作者：
Ishihama, Akira
影响因子：
14.9
作者：
Dame, RT;Wyman, C;Goosen, N
通讯作者：
Goosen, N
影响因子：
3.2
作者：
BALL, CA;OSUNA, R;JOHNSON, RC
通讯作者：
JOHNSON, RC
DOI：
10.1073/pnas.0506687102
发表时间：
2005-12-06
影响因子：
11.1
作者：
Grainger, DC;Hurd, D;Busby, SJW
通讯作者：
Busby, SJW
影响因子：
2.1
作者：
Azam, TA;Hiraga, S;Ishihama, A
通讯作者：
Ishihama, A