Simple Culture Methods and Treatment to Study Hormonal Regulation of Ovule Development.
Simple Culture Methods and Treatment to Study Hormonal Regulation of Ovule Development.
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研究胚珠发育激素调节的简单培养方法和处理
DOI：
10.3389/fpls.2018.00784
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发表时间：
2018
影响因子：
5.6
通讯作者：
Lin WH
中科院分区：
文献类型：
--
作者：
Li BF;Yu SX;Hu LQ;Zhang YJ;Zhai N;Xu L;Lin WH
胚珠发育是高等植物生殖发育的重要过程之一，决定着种子的质量和数量。植物激素在这一过程中起着关键作用，因为激素信号丧失的突变体表现出缺陷的胚珠表型和降低的育性。然而，激素对胚珠发育的直接影响难以区分，因为它是生殖发育的一部分，而缺陷表型可能是营养发育缺陷后的间接影响。激素处理是研究激素调控胚珠发育的一种直接方法，但胚珠嵌入在多层花器官中，传统的激素（或抑制剂）处理方法存在各种局限性。我们开发了一种简单的方法来处理活植物的花朵，将花序尖端浸入倒置管中的溶液中。我们还开发了一种特殊的系统来培养和处理切除的花/雌蕊。这些方法将有助于研究激素对胚珠发育的调节作用。我们提供了油菜素内酯（BR）和BR生物合成抑制剂治疗的例子。利用新方法培养和处理植物材料，观察野生型胚珠的形态和荧光信号，监测胚珠发育过程。结果表明，溴化反应促进胚珠发育，新方法高效、可重复性好。
Ovule development is one of the most important processes in the reproductive development of higher plants and is a determinant of seed quality and quantity. Phytohormones play key roles in this process since loss-of-function mutants in hormone signaling show defective ovule phenotypes and reduced fertility. However, it is difficult to distinguish the direct effects of hormones on ovule development because it is parts of reproductive development and the defective phenotypes would be the indirect effects following the defective vegetative development. The treatment of hormones is a direct method to investigate the hormonal regulation of ovule development, but ovule is embedded inside several layers of floral organs, and traditional methods for hormone (or inhibitor) treatments have various limitations. We have developed simple methods to apply treatments to the flowers in a living plant, where an inflorescence apex is immersed into a solution in an inverted tube. We have also developed a specific system to culture and treat excised flowers/pistils. These procedures will be useful for research on the hormonal regulation of ovule development. We provide examples of how treatments with brassinosteroids (BR) and BR biosynthesis inhibitor. We cultured and treated plant materials using our newly developed methods, and observed the morphology of wild type ovules and fluorescence signals in a marker line to monitor the progress of ovule development. The results demonstrate BR promotes ovule development and our new methods are efficient and repeatable.
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影响因子：
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作者：
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DOI：
10.1002/reg2.71
发表时间：
2017-02
期刊：
Regeneration (Oxford, England)
影响因子：
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作者：
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通讯作者：
Melnyk CW