Disrupting the Acyl Carrier Protein/SpoT interaction in vivo: identification of ACP residues involved in the interaction and consequence on growth.
Disrupting the Acyl Carrier Protein/SpoT interaction in vivo: identification of ACP residues involved in the interaction and consequence on growth.
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DOI：
10.1371/journal.pone.0036111
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Bouveret E
中科院分区：
文献类型：
--
作者：
Angelini S;My L;Bouveret E
在细菌中，酰基载体蛋白（ACP）是脂肪酸生物合成的核心辅助因子。它携带酰基链延伸，因此必须连续与该途径的所有酶相互作用。然而，ACP 还与多种不相关功能的蛋白质相互作用。其中，与 SpoT 的相互作用被认为参与调节细胞中 ppGpp 水平，以响应脂肪酸合成抑制。为了更好地理解这一机制，我们通过细菌双杂交筛选了无法在体内与SpoT相互作用但仍具有脂肪酸合成功能的ACP突变体。所选突变的位置表明 ACP 的螺旋 II 负责与 SpoT 相互作用。这表明了一种类似于脂肪酸合成酶的识别机制。一致地，ACP 与脂肪酸合成酶的细菌双杂交体测试的相互作用也受到阻止与 SpoT 相互作用的突变的影响。然而，有趣的是，相应的突变菌株是可行的，并且一种突变菌株的表型表明生长调节存在缺陷。
In bacteria, Acyl Carrier Protein (ACP) is the central cofactor for fatty acid biosynthesis. It carries the acyl chain in elongation and must therefore interact successively with all the enzymes of this pathway. Yet, ACP also interacts with proteins of diverse unrelated function. Among them, the interaction with SpoT has been proposed to be involved in regulating ppGpp levels in the cell in response to fatty acid synthesis inhibition. In order to better understand this mechanism, we screened for ACP mutants unable to interact with SpoT in vivo by bacterial two-hybrid, but still functional for fatty acid synthesis. The position of the selected mutations indicated that the helix II of ACP is responsible for the interaction with SpoT. This suggested a mechanism of recognition similar to one used for the enzymes of fatty acid synthesis. Consistently, the interactions tested by bacterial two-hybrid of ACP with fatty acid synthesis enzymes were also affected by the mutations that prevented the interaction with SpoT. Yet, interestingly, the corresponding mutant strains were viable, and the phenotypes of one mutant suggested a defect in growth regulation.
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影响因子：
3.7
作者：
Atkinson GC;Tenson T;Hauryliuk V
通讯作者：
Hauryliuk V
影响因子：
4.8
作者：
De lay, Nicholas R.;Cronan, John E.
通讯作者：
Cronan, John E.
DOI：
10.1016/s0969-2126(00)00178-7
发表时间：
2000-08-15
期刊：
STRUCTURE WITH FOLDING & DESIGN
影响因子：
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作者：
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通讯作者：
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影响因子：
3.6
作者：
den Blaauwen, T;Lindqvist, A;Nanninga, N
通讯作者：
Nanninga, N
影响因子：
3.4
作者：
Battesti, Aurelia;Bouveret, Emmanuelle
通讯作者：
Bouveret, Emmanuelle