L-Theanine Regulates the Abundance of Amino Acid Transporters in Mice Duodenum and Jejunum via the mTOR Signaling Pathway.
L-Theanine Regulates the Abundance of Amino Acid Transporters in Mice Duodenum and Jejunum via the mTOR Signaling Pathway.
复制标题
L-茶氨酸通过 mTOR 信号通路调节小鼠十二指肠和空肠中氨基酸转运蛋白的丰度
作者：
Liu K;Peng Y;Lin L;Gong Z;Xiao W;Li Y
肠道是吸收氨基酸的关键器官。L-茶氨酸（LTA）是谷氨酰胺的结构类似物，也是茶（Camellia sinensis）中发现的特征性非蛋白质氨基酸，调节脂质和蛋白质代谢。本研究探讨了LTA在肠道氨基酸吸收、蛋白质合成中的作用及其机制。总的来说，我们的研究结果表明，LTA补充不仅影响血清碱性磷酸酶（AKP），总蛋白（TP）和尿素氮（BUN）水平，而且还上调氨基酸转运蛋白（EAAT 3，EAAT 1，4F 2 hc，y+ LAT 1，CAT 1，ASCT 2和B 0AT 1）的mRNA和蛋白表达，并激活mTOR信号通路。下游S6和S6 K1蛋白受到调节，氨基酸转运蛋白的表达受到调节。这些发现表明，LTA通过上调AAT表达、激活mTOR信号通路和磷酸化mTOR下游蛋白S6和S6 K1来增加肠道AA吸收、促进蛋白质代谢并增加氮利用。
The intestine is a key organ for the absorption of amino acids. L-theanine (LTA) is a structural analog of glutamine and a characteristic non-protein amino acid found in tea (Camellia sinensis) that regulates lipid and protein metabolism. The present study explored the role of LTA in intestinal amino acid absorption, protein synthesis, and its mechanisms. Overall, our findings suggest that LTA supplementation not only affects serum alkaline phosphatase (AKP), total protein (TP), and urea nitrogen (BUN) levels, but it also upregulates the mRNA and protein expression of amino acid transporters (EAAT3, EAAT1, 4F2hc, y+LAT1, CAT1, ASCT2, and B0AT1), and activates the mTOR signaling pathway. The downstream S6 and S6K1 proteins are regulated, and the expression of amino acid transporters is regulated. These findings suggest that LTA increases intestinal AA absorption, promotes protein metabolism, and increases nitrogen utilization by upregulating AAT expression, activating the mTOR signaling pathway, and phosphorylating the mTOR downstream proteins S6 and S6K1.
DOI：
10.3390/molecules26227002
发表时间：
2021-11-19
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Zhang L;Ma M;Li Z;Zhang H;He X;Song Z
通讯作者：
Song Z
影响因子：
2.7
作者：
Takarada T;Nakamichi N;Nakazato R;Kakuda T;Kokubo H;Ikeno S;Nakamura S;Kuramoto N;Hinoi E;Yoneda Y
通讯作者：
Yoneda Y