Protective Effects of L-Theanine on IPEC-J2 Cells Growth Inhibition Induced by Dextran Sulfate Sodium via p53 Signaling Pathway.
Protective Effects of L-Theanine on IPEC-J2 Cells Growth Inhibition Induced by Dextran Sulfate Sodium via p53 Signaling Pathway.
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DOI：
10.3390/molecules26227002
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发表时间：
2021-11-19
期刊：
影响因子：
--
通讯作者：
Song Z
中科院分区：
文献类型：
--
作者：
Zhang L;Ma M;Li Z;Zhang H;He X;Song Z
L-茶氨酸是一种存在于茶叶中的非蛋白质氨基酸，由于其多种生物活性，已被广泛用作饮料或食品中的安全食品添加剂。本研究的目的是利用DSS诱导的猪小肠上皮细胞（IPEC-J2）模型，通过分子和代谢的方法，揭示L-茶氨酸的体外胃肠道保护作用。结果表明，2.5%葡聚糖硫酸钠（DSS）处理可抑制IPEC-J2细胞增殖，阻断细胞周期的正常运行，而L-茶氨酸预处理可明显保持这些趋势，发挥保护作用。L-茶氨酸预处理还上调DSS处理的细胞中EGF、CDC 2、FGF 2、Rb基因的表达，下调p53、p21增殖相关mRNA的表达，同时伴有p53信号通路的抑制。同时，代谢组学分析显示，L-茶氨酸和DSS处理的IPEC-J2细胞具有不同的代谢组学特征，参与嘧啶代谢和氨基酸代谢的关键代谢物发生了显著变化，这两种代谢物在核苷酸代谢中起着重要作用。综上所述，L-茶氨酸通过促进细胞增殖和调节代谢紊乱对DSS诱导的IPEC-J2细胞具有有益的保护作用。
L-theanine is a nonprotein amino acid found in tea leaves and has been widely used as a safe food additive in beverages or foods because of its varied bioactivities. The aim of this study was to reveal the in vitro gastrointestinal protective effects of L-theanine in DSS-induced intestinal porcine enterocyte (IPEC-J2) cell models using molecular and metabolic methods. Results showed that 2.5% dextran sulfate sodium (DSS) treatment inhibited the cell proliferation of IPEC-J2 and blocked the normal operation of the cell cycle, while L-theanine pretreatment significantly preserved these trends to exert protective effects. L-theanine pre-treatment also up-regulated the EGF, CDC2, FGF2, Rb genes and down-regulated p53, p21 proliferation-related mRNA expression in DSS-treated cells, in accompany with p53 signaling pathway inhibition. Meanwhile, metabolomics analysis revealed that L-theanine and DSS treated IPEC-J2 cells have different metabolomic profiles, with significant changes in the key metabolites involved in pyrimidine metabolism and amino acid metabolism, which play an important role in nucleotide metabolism. In summary, L-theanine has a beneficial protection in DSS-induced IPEC-J2 cells via promoting proliferation and regulating metabolism disorders.
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