Methanolobus use unspecific methyltransferases to produce methane from dimethylsulphide in Baltic Sea sediments.
Methanolobus use unspecific methyltransferases to produce methane from dimethylsulphide in Baltic Sea sediments.
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DOI：
10.1186/s40168-023-01720-w
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发表时间：
2024-01-03
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影响因子：
15.5
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中科院分区：
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在缺氧的沿海和海洋沉积物中，甲基化化合物的降解是产生甲烷（一种强大的温室气体）的主要途径。二甲基硫（DMS）是环境中最丰富的生物有机硫化合物，也是缺氧沉积物中大量甲基化的甲烷生成物。然而，对微生物多样性驱动DMS依赖的甲烷生成的理解是有限的，并且环境中该过程的代谢途径仍然未被探索。为了解决这个问题，我们使用缺氧培养，扩增子测序，基因组为中心的宏基因组学和微咸沉积物的metatranscriptomics收集沿着波罗的海的深度剖面不同的硫酸盐浓度。我们确定Methanolobus为占主导地位的甲基营养型产甲烷菌在我们所有的DMS修正的沉积物培养（61-99%），无论其硫酸盐浓度。我们还表明，当沉积物样本与DMS一起孵育时，来自Methanolobus的mtt和mta基因（三甲基胺甲基转移酶和甲醇甲基转移酶）高度表达。此外，我们在元转录组、宏基因组或Methanolobus MAG中没有发现mtsA和mtsB（甲基硫-甲基转移酶），而在所选样品中发现mtsD和mtsF低2-3个数量级。我们的研究表明，Methanolobus属可能是在微咸水波罗的海沉积物中的DMS厌氧降解的关键球员。这也是第一项分析环境中厌氧DMS降解代谢途径的研究，并表明DMS的甲基营养型甲烷生产可能不需要以前接受的底物特异性甲基转移酶。这突出了缺氧沉积物中甲烷生产关键酶的多功能性，这将对全球温室气体预算和甲烷循环产生重大影响。视频摘要在线版本包含补充材料，可通过10. 1186/s40168-023-01720-w获取。
In anoxic coastal and marine sediments, degradation of methylated compounds is the major route to the production of methane, a powerful greenhouse gas. Dimethylsulphide (DMS) is the most abundant biogenic organic sulphur compound in the environment and an abundant methylated compound leading to methane production in anoxic sediments. However, understanding of the microbial diversity driving DMS-dependent methanogenesis is limited, and the metabolic pathways underlying this process in the environment remain unexplored. To address this, we used anoxic incubations, amplicon sequencing, genome-centric metagenomics and metatranscriptomics of brackish sediments collected along the depth profile of the Baltic Sea with varying sulphate concentrations. We identified Methanolobus as the dominant methylotrophic methanogens in all our DMS-amended sediment incubations (61–99%) regardless of their sulphate concentrations. We also showed that the mtt and mta genes (trimethylamine- and methanol-methyltransferases) from Methanolobus were highly expressed when the sediment samples were incubated with DMS. Furthermore, we did not find mtsA and mtsB (methylsulphide-methyltransferases) in metatranscriptomes, metagenomes or in the Methanolobus MAGs, whilst mtsD and mtsF were found 2–3 orders of magnitude lower in selected samples. Our study demonstrated that the Methanolobus genus is likely the key player in anaerobic DMS degradation in brackish Baltic Sea sediments. This is also the first study analysing the metabolic pathways of anaerobic DMS degradation in the environment and showing that methylotrophic methane production from DMS may not require a substrate-specific methyltransferase as was previously accepted. This highlights the versatility of the key enzymes in methane production in anoxic sediments, which would have significant implications for the global greenhouse gas budget and the methane cycle. Video Abstract The online version contains supplementary material available at 10.1186/s40168-023-01720-w.
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6.4
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Clum A;Huntemann M;Bushnell B;Foster B;Foster B;Roux S;Hajek PP;Varghese N;Mukherjee S;Reddy TBK;Daum C;Yoshinaga Y;O'Malley R;Seshadri R;Kyrpides NC;Eloe-Fadrosh EA;Chen IA;Copeland A;Ivanova NN
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SHOOTER, D
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Bruchert, V.