Human protamine-1 as an MRI reporter gene based on chemical exchange.
Human protamine-1 as an MRI reporter gene based on chemical exchange.
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DOI：
10.1021/cb400617q
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发表时间：
2014-01-17
影响因子：
4
通讯作者：
Gilad, Assaf A.
中科院分区：
文献类型：
--
作者：
Bar-Shir, Amnon;Liu, Guanshu;Chan, Kannie W. Y.;Oskolkov, Nikita;Song, Xiaolei;Yadav, Nirbhay N.;Walczak, Piotr;McMahon, Michael T.;van Zijl, Peter C. M.;Bulte, Jeff W. M.;Gilad, Assaf A.
基因工程记者已经彻底改变了许多生物过程的理解。基于MRI的报告基因可以显著提高我们监测动态基因表达的能力，并允许将亚细胞遗传信息与高分辨率解剖图像配准。我们已经开发了一个生物相容性的MRI报告基因的基础上，人类的鱼精蛋白-1（hPRM 1）。富含精氨酸的hPRM 1（47%精氨酸残基）基于化学交换饱和转移（CEST）对比机制产生高MRI对比度。使用微波辅助技术完全合成了51个氨基酸长的hPRM 1蛋白，并将该蛋白的CEST特征与其他基于CEST的造影剂进行了比较。细菌和人细胞都被工程化以表达优化的hPRM 1基因，并且与对照相比显示出更高的CEST对比度。与野生型活细胞相比，表达hPRM 1报告基因并嵌入三维培养物中的活细胞也产生更高的CEST对比度。
Genetically engineered reporters have revolutionized the understanding of many biological processes. MRI-based reporter genes can dramatically improve our ability to monitor dynamic gene expression and allow coregistration of subcellular genetic information with high-resolution anatomical images. We have developed a biocompatible MRI reporter gene based on a human gene, the human protamine-1 (hPRM1). The arginine-rich hPRM1 (47% arginine residues) generates high MRI contrast based on the chemical exchange saturation transfer (CEST) contrast mechanism. The 51 amino acid-long hPRM1 protein was fully synthesized using microwave-assisted technology, and the CEST characteristics of this protein were compared to other CEST-based contrast agents. Both bacterial and human cells were engineered to express an optimized hPRM1 gene and showed higher CEST contrast compared to controls. Live cells expressing the hPRM1 reporter gene, and embedded in three-dimensional culture, also generated higher CEST contrast compared to wild-type live cells.
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影响因子：
3.3
作者：
McMahon, Michael T.;Gilad, Assaf A.;DeLiso, Marco A.;Berman, Stacey D. Cromer;Bulte, Jeff W. M.;van Zijl, Peter C. M.
通讯作者：
van Zijl, Peter C. M.
影响因子：
15
作者：
Liu, Guanshu;Liang, Yajie;Bar-Shir, Amnon;Chan, Kannie W. Y.;Galpoththawela, Chulani S.;Bernard, Segun M.;Tse, Terence;Yadav, Nirbhay N.;Walczak, Piotr;McMahon, Michael T.;Bulte, Jeff W. M.;van Zijl, Peter C. M.;Gilad, Assaf A.
通讯作者：
Gilad, Assaf A.
影响因子：
3.3
作者：
Liu, Guanshu;Li, Yuguo;Pagel, Mark D.
通讯作者：
Pagel, Mark D.
影响因子：
3.3
作者：
Snoussi, K;Bulte, JWM;van Zijl, PCM
通讯作者：
van Zijl, PCM
影响因子：
2.2
作者：
Ward, KM;Aletras, AH;Balaban, RS
通讯作者：
Balaban, RS