New "multicolor" polypeptide diamagnetic chemical exchange saturation transfer (DIACEST) contrast agents for MRI.
New "multicolor" polypeptide diamagnetic chemical exchange saturation transfer (DIACEST) contrast agents for MRI.
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DOI：
10.1002/mrm.21683
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发表时间：
2008-10
影响因子：
3.3
通讯作者：
van Zijl, Peter C. M.
中科院分区：
文献类型：
--
作者：
McMahon, Michael T.;Gilad, Assaf A.;DeLiso, Marco A.;Berman, Stacey D. Cromer;Bulte, Jeff W. M.;van Zijl, Peter C. M.
根据化学交换饱和转移（CEST）机制，选择了33个原型多肽的阵列作为推定的对比剂。产生这种对比的羟基质子，并在11.7T的情况下进行了cest颜色。 （赖氨酸，精氨酸，苏氨酸或系列）基于这些可交换质子的单独共振频率，可以通过改变氨基酸序列来微调CEST效应的大小根据它们的不同化学移位（“颜色”）。适用于多种生物学应用，例如设计用于成像细胞的MR报告基因并在同一MR图像中区分多个靶标。
An array of 33 prototype polypeptides was examined as putative contrast agents that can be distinguished from each other based on the Chemical Exchange Saturation Transfer (CEST) mechanism. These peptides were chosen based on predictions of the chemical exchange rates of exchangeable amide, amine, and hydroxyl protons that produce this contrast, and tested at 11.7T for their CEST suitability. Artificial colors were assigned to particular amino acid units (lysine, arginine, threonine or serine) based on the separate resonance frequencies of these exchangeable protons. The magnitude of the CEST effect could be fine-tuned by altering the amino acid sequence, and these three exchangeable groups could be distinguished in an MR phantom based on their different chemical shifts (“colors”). These new DIACEST agents possess a wide range of electrostatic charges, compositions, and protein stabilities in vivo, making them potentially suitable for a variety of biological applications such as designing MR reporter genes for imaging cells and distinguishing multiple targets within the same MR image.
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影响因子：
3.3
作者：
Snoussi, K;Bulte, JWM;van Zijl, PCM
通讯作者：
van Zijl, PCM
影响因子：
2.9
作者：
BJELLQVIST, B;HUGHES, GJ;HOCHSTRASSER, D
通讯作者：
HOCHSTRASSER, D
影响因子：
2.2
作者：
Ward, KM;Aletras, AH;Balaban, RS
通讯作者：
Balaban, RS
影响因子：
4.4
作者：
FORSEN, S;HOFFMAN, RA
通讯作者：
HOFFMAN, RA
影响因子：
1.6
作者：
Lewis, JD;Song, Y;Ausió, J
通讯作者：
Ausió, J