Homoeolog-specific activation of genes for heat acclimation in the allopolyploid grass Brachypodium hybridum.
Homoeolog-specific activation of genes for heat acclimation in the allopolyploid grass Brachypodium hybridum.
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DOI：
10.1093/gigascience/giy020
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发表时间：
2018-04-01
期刊：
影响因子：
9.2
通讯作者：
Mochida K
中科院分区：
文献类型：
--
作者：
Takahagi K;Inoue K;Shimizu M;Uehara-Yamaguchi Y;Onda Y;Mochida K
异源多倍体植物通常表现出比它们的祖先更广泛的环境耐受性；这被认为是由于多个不同的基因组以固定的杂合性合并而成的。复杂的同源基因表达可能在进化上有利于异源多倍体植物的适应。尽管之前的多项研究报告了同源基因在异源多倍体物种中的表达，但还没有明确的例子表明同源基因在适应长期胁迫条件下的功能。我们发现，异源多倍体杂交草及其祖先短穗短柱草表现出长期的耐热胁迫能力，而不同于它的另一个祖先短穗短柱草。为了了解杂交藻的生理特性，比较了在正常和高温胁迫条件下生长的3种短柄蕨属植物的转录组。我们发现，在正常条件下，大约26%的同源群体和大约38%的同源群体的表达模式分别转变为非加性表达和非祖先表达。此外，我们还发现B.disachyon在正常和热胁迫条件下表现出相似的表达模式，而B.杂交和B.stacei在胁迫3天后显著改变了它们的转录组，从B.stacei遗传的同源基因可能参与了B.杂交豆类对热的转录胁迫反应。在热暴露15天后，杂交假单胞菌和假单胞菌保持与正常条件下相似的转录状态。这些结果表明，在长期的热胁迫下，来自球孢杆菌的同源物对热的早期反应有助于细胞内环境的稳定。我们的结果为同源转录组的不同调控事件提供了洞察力，这些调控事件与异源多倍体植物的逆境适应有关。
Allopolyploid plants often show wider environmental tolerances than their ancestors; this is expected to be due to the merger of multiple distinct genomes with a fixed heterozygosity. The complex homoeologous gene expression could have been evolutionarily advantageous for the adaptation of allopolyploid plants. Despite multiple previous studies reporting homoeolog-specific gene expression in allopolyploid species, there are no clear examples of homoeolog-specific function in acclimation to a long-term stress condition. We found that the allopolyploid grass Brachypodium hybridum and its ancestor Brachypodium stacei show long-term heat stress tolerance, unlike its other ancestor, Brachypodium distachyon. To understand the physiological traits of B. hybridum, we compared the transcriptome of the 3 Brachypodium species grown under normal and heat stress conditions. We found that the expression patterns of approximately 26% and approximately 38% of the homoeolog groups in B. hybridum changed toward nonadditive expression and nonancestral expression, respectively, under normal condition. Moreover, we found that B. distachyon showed similar expression patterns between normal and heat stress conditions, whereas B. hybridum and B. stacei significantly altered their transcriptome in response to heat after 3 days of stress exposure, and homoeologs that were inherited from B. stacei may have contributed to the transcriptional stress response to heat in B. hybridum. After 15 days of heat exposure, B. hybridum and B. stacei maintained transcriptional states similar to those under normal conditions. These results suggest that an earlier response to heat that was specific to homoeologs originating from B. stacei contributed to cellular homeostasis under long-term heat stress in B. hybridum. Our results provide insights into different regulatory events of the homoeo-transcriptome that are associated with stress acclimation in allopolyploid plants.
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影响因子：
5.6
作者：
Driedonks N;Xu J;Peters JL;Park S;Rieu I
通讯作者：
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DOI：
10.1073/pnas.0900906106
发表时间：
2009-04-07
影响因子：
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