Snail1 expression in colorectal cancer and its correlation with clinical and pathological parameters.
Snail1 expression in colorectal cancer and its correlation with clinical and pathological parameters.
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DOI：
10.1186/1471-2407-13-145
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发表时间：
2013-03-22
期刊：
影响因子：
3.8
通讯作者：
Knoefel WT
中科院分区：
文献类型：
--
作者：
Kroepil F;Fluegen G;Vallböhmer D;Baldus SE;Dizdar L;Raffel AM;Hafner D;Stoecklein NH;Knoefel WT
Snail 1是E-cadherin的转录调节因子。E-cadherin的缺失似乎是上皮间质转化（EMT）过程中的关键步骤。EMT在许多肿瘤中启动侵袭和增殖。已知Snail 1的过表达与几种实体瘤的不良结局相关。本研究的目的是分析其在结直肠癌中的表达谱和预后意义。本研究使用了包含来自251名患者的石蜡包埋的原发性结直肠癌（CRC）组织样本的组织微阵列（TMA）。Snail 1和E-cadherin的表达通过免疫组化评估在不同的肿瘤隔室，相应的淋巴结转移和正常结肠粘膜。染色强度根据Remmele评分（标准化评分系统）以及Blechschallen等人建立的半定量评分进行分类。在76%的CRC中观察到Snail 1表达。在87%的CRC中观察到E-钙粘蛋白的丢失。Snail 1阳性肿瘤与Snail 1阳性淋巴结转移显著相关（p=0.03）。E-cadherin的缺失与Snail 1的表达无明显相关性，N分期或分级与Snail 1的表达无明显相关性。Kaplan-Meier生存分析表明Snail 1表达对总生存期无预后影响。在大多数结直肠癌中可检测到Snail 1表达，但与E-钙粘蛋白丢失、临床病理特征或总生存期无显著相关性。所观察到的E-钙粘蛋白的损失可以解释为其他重要的EMT途径，如Wnt信号级联的影响。
Snail1 is a transcription regulator of E-cadherin. The loss of E-cadherin seems to be a crucial step in the process of Epithelial-mesenchymal transition (EMT). EMT initiates invasion and proliferation in many tumours. Overexpression of Snail1 is known to be associated with poor outcome in several solid tumours. The aim of this study was to analyse its expression profile and prognostic significance in colorectal cancer. Tissue microarrays (TMA) containing paraffin-embedded primary colorectal cancer (CRC) tissue samples from 251 patients were used in this study. The expression of Snail1 and E-cadherin was assessed by immunohistochemistry in different tumour compartments, corresponding lymph node metastases and normal colonic mucosa. Intensity of staining was classified according to the Remmele score (standardized scoring system) as well as the semiquantitative score established by Blechschmidt et al. Snail1 expression was observed in 76% of the CRC. Loss of E-cadherin was noted in 87% of the CRC. Snail1 positive tumours were significantly correlated with Snail1 positive lymph node metastases (p=0.03). There was no significant correlation between loss of E-cadherin and Snail1 expression, or between N-stage or grading and Snail1 expression. Kaplan-Meier survival analysis identified no prognostic impact of Snail1 expression on overall survival. Snail1 expression was detectable in most of the CRC but showed no significant association with E-cadherin loss, clinical pathological characteristics or overall survival. The observed loss of E-cadherin could be explained by effects of other important EMT pathways, such as the Wnt-signalling cascade.
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影响因子：
5.3
作者：
Hipp S;Walch A;Schuster T;Losko S;Laux H;Bolton T;Höfler H;Becker KF
通讯作者：
Becker KF
DOI：
10.1186/bcr3236
发表时间：
2012-07-27
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Fredlund E;Staaf J;Rantala JK;Kallioniemi O;Borg A;Ringnér M
通讯作者：
Ringnér M
影响因子：
5.6
作者：
Bezdekova M;Brychtova S;Sedlakova E;Langova K;Brychta T;Belej K
通讯作者：
Belej K
影响因子：
2.9
作者：
Kawashima, Atsunari;Takayama, Hitoshi;Tsujimura, Akira
通讯作者：
Tsujimura, Akira
影响因子：
2.8
作者：
Brabletz, T;Jung, A;Kirchner, T
通讯作者：
Kirchner, T