The diversity of the Limnohabitans genus, an important group of freshwater bacterioplankton, by characterization of 35 isolated strains.
The diversity of the Limnohabitans genus, an important group of freshwater bacterioplankton, by characterization of 35 isolated strains.
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DOI：
10.1371/journal.pone.0058209
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Šimek K
中科院分区：
文献类型：
--
作者：
Kasalický V;Jezbera J;Hahn MW;Šimek K
Limnohabitans属的细菌，更确切地说是R-BT谱系，由于其高的基质吸收和生长速率，在藻类来源的基质上生长以及来自噬菌性的高死亡率，在淡水浮游细菌群落中具有突出的作用。此外，由于它们的平均细胞体积一般较大，与典型的浮游细菌细胞相比，它们对总浮游细菌生物量的贡献比例过高。在这里，我们提出了35个细菌菌株隶属于新分离的11个非酸性欧洲淡水栖息地的Limnohabitans属的遗传，形态和生理生态特性。低的遗传多样性表明，由以前的研究使用核糖体SSU基因的高度对比与令人惊讶的丰富的形态和不同的模式，在底物利用的分离菌株。因此，16 S和23 S rRNA基因之间的基因间隔区被成功地测试作为一个精细尺度的标记，描绘个别谱系，甚至基因型。为了进一步的研究，我们建议将Limnohabitans属划分为五个谱系（暂时命名为LimA，LimB，LimC，LimD和LimE），以及最多样化的谱系LimC中的其他亚谱系。这种划分是由分离菌株的形态学支持的，这些形态学预先确定了它们在生态学上的巨大差异。
Bacteria of the genus Limnohabitans, more precisely the R-BT lineage, have a prominent role in freshwater bacterioplankton communities due to their high rates of substrate uptake and growth, growth on algal-derived substrates and high mortality rates from bacterivory. Moreover, due to their generally larger mean cell volume, compared to typical bacterioplankton cells, they contribute over-proportionally to total bacterioplankton biomass. Here we present genetic, morphological and ecophysiological properties of 35 bacterial strains affiliated with the Limnohabitans genus newly isolated from 11 non-acidic European freshwater habitats. The low genetic diversity indicated by the previous studies using the ribosomal SSU gene highly contrasted with the surprisingly rich morphologies and different patterns in substrate utilization of isolated strains. Therefore, the intergenic spacer between 16S and 23S rRNA genes was successfully tested as a fine-scale marker to delineate individual lineages and even genotypes. For further studies, we propose the division of the Limnohabitans genus into five lineages (provisionally named as LimA, LimB, LimC, LimD and LimE) and also additional sublineages within the most diversified lineage LimC. Such a delineation is supported by the morphology of isolated strains which predetermine large differences in their ecology.
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影响因子：
4.2
作者：
Galperin MY
通讯作者：
Galperin MY
影响因子：
4.4
作者：
Crump, BC;Kling, GW;Hobbie, JE
通讯作者：
Hobbie, JE
影响因子：
4.4
作者：
Boenigk, J;Stadler, P;Hahn, MW
通讯作者：
Hahn, MW
DOI：
10.1099/ijs.0.022384-0
发表时间：
2010-12
影响因子：
2.8
作者：
Hahn MW;Kasalický V;Jezbera J;Brandt U;Šimek K
通讯作者：
Šimek K
影响因子：
4.2
作者：
Hörtnagl P;Pérez MT;Zeder M;Sommaruga R
通讯作者：
Sommaruga R