Nitric oxide-mediated dispersal in single- and multi-species biofilms of clinically and industrially relevant microorganisms.
Nitric oxide-mediated dispersal in single- and multi-species biofilms of clinically and industrially relevant microorganisms.
复制标题
DOI：
10.1111/j.1751-7915.2009.00098.x
复制
发表时间：
2009-05
影响因子：
5.7
通讯作者：
Kjelleberg S
中科院分区：
文献类型：
--
作者：
Barraud N;Storey MV;Moore ZP;Webb JS;Rice SA;Kjelleberg S
诱导生物膜分散的策略由于其预防生物膜形成和生物膜相关感染的潜力而受到关注。一氧化氮（NO）是生物系统中的一种重要信使分子，被认为是铜绿假单胞菌生物膜中的一种信号分子。在本研究中，更广泛地评估了NO作为抗生物膜剂的使用。在相关微生物的单物种生物膜和来自水分配和处理系统的多物种生物膜上测试了各种NO供体，其浓度估计为产生皮摩尔和低纳摩尔范围内的NO水平。在所有评估的生物膜中观察到一氧化氮诱导的分散，总生物膜表面的平均减少为63%。此外，暴露于低剂量的NO的生物膜比未处理的生物膜更容易受到抗微生物处理。例如，与未处理的生物膜相比，用NO处理的生物膜中常规氯处理从水系统中去除多物种生物膜的功效增加了20倍。这些数据表明，与NO的组合治疗可以允许新的和改进的策略来控制生物膜，并在许多环境，工业和临床环境中具有广泛的应用。
Strategies to induce biofilm dispersal are of interest due to their potential to prevent biofilm formation and biofilm‐related infections. Nitric oxide (NO), an important messenger molecule in biological systems, was previously identified as a signal for dispersal in biofilms of the model organism Pseudomonas aeruginosa. In the present study, the use of NO as an anti‐biofilm agent more broadly was assessed. Various NO donors, at concentrations estimated to generate NO levels in the picomolar and low nanomolar range, were tested on single‐species biofilms of relevant microorganisms and on multi‐species biofilms from water distribution and treatment systems. Nitric oxide‐induced dispersal was observed in all biofilms assessed, and the average reduction of total biofilm surface was 63%. Moreover, biofilms exposed to low doses of NO were more susceptible to antimicrobial treatments than untreated biofilms. For example, the efficacy of conventional chlorine treatments at removing multi‐species biofilms from water systems was increased by 20‐fold in biofilms treated with NO compared with untreated biofilms. These data suggest that combined treatments with NO may allow for novel and improved strategies to control biofilms and have widespread applications in many environmental, industrial and clinical settings.
登录
查看更多内容
影响因子：
2.9
作者：
Delgado-Nixon, VM;Gonzalez, G;Gilles-Gonzalez, MA
通讯作者：
Gilles-Gonzalez, MA
影响因子：
17.1
作者：
Hetrick, Evan M.;Shin, Jae Ho;Schoenfisch, Mark H.
通讯作者：
Schoenfisch, Mark H.
DOI：
10.1164/ajrccm.165.7.2105032
发表时间：
2002-04-01
影响因子：
24.7
作者：
Snyder, AH;McPherson, ME;Gaston, B
通讯作者：
Gaston, B
影响因子：
3.2
作者：
GIVSKOV, M;OLSEN, L;MOLIN, S
通讯作者：
MOLIN, S
影响因子：
3.1
作者：
Darling, KEA;Evans, TJ
通讯作者：
Evans, TJ