Anxiolytic effects of 5-HT₁A receptors and anxiogenic effects of 5-HT₂C receptors in the amygdala of mice.
Anxiolytic effects of 5-HT₁A receptors and anxiogenic effects of 5-HT₂C receptors in the amygdala of mice.
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DOI：
10.1016/j.neuropharm.2011.09.002
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发表时间：
2012-01
影响因子：
4.7
通讯作者：
Wang, Jing
中科院分区：
文献类型：
--
作者：
Li, Qian;Luo, Tian;Jiang, Xue;Wang, Jing
本研究旨在验证杏仁核中的5 - 羟色胺1A（5 - HT1A）和5 - 羟色胺2C（5 - HT2C）受体在焦虑行为调节中起重要作用这一假设。我们采用重组腺病毒方法，通过操控杏仁核中5 - HT1A和5 - HT2C受体的表达，来研究类似焦虑行为的变化。将含有5 - HT1A启动子控制的5 - HT1A反义序列或5 - HT2C启动子控制的5 - HT2C正义序列的重组腺病毒注入杏仁核。通过高架十字迷宫（EPM）实验和旷场实验来测定类似焦虑行为和自发活动。杏仁核中5 - HT1A受体表达的降低，显著减少了在高架十字迷宫开放臂停留的时间以及在旷场中央停留的时间。在高架十字迷宫开放臂停留时间百分比的降低与中央杏仁核中5 - HT1A受体的密度呈负相关。另一方面，5 - HT2C受体表达的增加，减少了在高架十字迷宫开放臂停留的时间以及在旷场中央停留的时间。在高架十字迷宫开放臂停留时间和移动距离的减少与杏仁核基底外侧核中5 - HT2C受体的密度相关。这些数据表明，杏仁核5 - HT1A受体产生抗焦虑作用，而5 - HT2C受体产生致焦虑作用。综上所述，本研究结果证明了杏仁核5 - HT1A和5 - HT2C受体在类似焦虑行为调节中的重要作用。
The aim of the present study is to test a hypothesis that 5-HT1A and 5-HT2C receptors in the amygdala play an important role in the regulation of anxiety behaviors. We examined alterations in anxiety-like behaviors after manipulation of the expression of 5-HT1A and 5-HT2C receptors in the amygdala using recombinant adenovirus approaches. Recombinant adenoviruses containing a 5-HT1A promoter-controlled 5-HT1A antisense sequence or a 5-HT2C promoter-controlled 5-HT2C sense sequence were injected into the amygdala. Elevated plus maze (EPM) and open field tests were conducted to determine anxiety-like behavior and locomotor activity. Reductions in the expression of 5-HT1A receptors in the amygdala significantly attenuated the time spent in the open arms of EPM and time spent in the center of an open field. Reduction in the percent of time spent in the open arms of EPM is negatively correlated with the density of 5-HT1A receptors in the central amygdala. On the other hand, increased expression of 5-HT2C receptors reduced the time spent in the open arms of EPM and time spent in the center of an open field. The reductions in the time spent and distance traveled in the open arms of EPM were correlated to the density of 5-HT2C receptors in the basolateral nucleus of amygdala. These data suggest that amygdaloid 5-HT1A receptors produce anxiolytic and 5-HT2C receptors produce anxiogenic effects. Together, the present results demonstrate the important role of the amygdaloid 5-HT1A and 5-HT2C receptors in the regulation of anxiety-like behaviors.
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影响因子：
10.6
作者：
Christianson, John P.;Ragole, Thomas;Amat, Jose;Greenwood, Benjamin N.;Strong, Paul V.;Paul, Evan D.;Fleshner, Monika;Watkins, Linda R.;Maier, Steven F.
通讯作者：
Maier, Steven F.
DOI：
10.1017/s1461145701002632
发表时间：
2001-12-01
影响因子：
4.8
作者：
Bagdy, G;Graf, M;Kantor, S
通讯作者：
Kantor, S
影响因子：
5.3
作者：
Li, Q;Holmes, A;Garcia, F
通讯作者：
Garcia, F
影响因子：
3.4
作者：
Cruz, APM;Pinheiro, G;Landeira-Fernandez, J
通讯作者：
Landeira-Fernandez, J
影响因子：
8.2
作者：
Holmes, Andrew
通讯作者：
Holmes, Andrew