Gene delivery via DNA incorporation within a biomimetic apatite coating.
Gene delivery via DNA incorporation within a biomimetic apatite coating.
复制标题
DOI：
10.1016/j.biomaterials.2009.09.001
复制
发表时间：
2009-12
期刊：
影响因子：
14
通讯作者：
Kohn, David H.
中科院分区：
文献类型：
--
作者：
Luong, Linh N.;McFalls, Kristen M.;Kohn, David H.
Integrating inductivity with conductivity in a material may advance tissue engineering. An organic/inorganic hybrid was developed by incorporating plasmid DNA encoding for the β-gal gene complexed with Lipofectamine 2000® (DNA-Lipoplex) within apatite via coprecipitation. It is hypothesized that this system will result in enhanced transfection efficiency compared to DNA-Lipoplexes adsorbed to the mineral surface and DNA coprecipitated without Lipofectamine 2000®. PLGA films were cast onto glass slips and apatite and DNA were coprecipitated in modified simulated body fluid (mSBF). DNA-Lipoplex presence in mineral, DNA-Lipoplex stability (vs. coprecipitation time), and transfection efficiency (determined with C3H10T1/2 cells) as a function of coprecipitation time, DNA-Lipoplex concentration, and DNA incorporation method were studied. DNA-Lipoplex presence and spatial distribution on apatite were confirmed through fluorescence. Transfection efficiency was highest for 6 h of DNA- Lipoplex coprecipitation. Differences in transfection efficiency were found between the DNA concentrations, with the highest efficiency for coprecipitation being 40 μg/ml (p≤0.009 relative to other coprecipitation concentrations). Significant differences in transfection efficiency existed between incorporation methods (p<0.05) with the highest efficiency for DNA-Lipoplex coprecipitation. This hybrid material system not only integrates inductivity provided by the DNA and conductivity provided by the apatite, but it also has significant implications in non-viral gene delivery due to its ability to increase transfection efficiency.
登录
查看更多内容
影响因子：
5.1
作者：
Park, J;Ries, J;Schneider, H
通讯作者：
Schneider, H
影响因子：
10.8
作者：
Leong, KW;Mao, HQ;August, JT
通讯作者：
August, JT
影响因子：
41.2
作者：
Kong, HJ;Liu, JD;Mooney, DJ
通讯作者：
Mooney, DJ
影响因子：
14.9
作者：
Jordan, M;Schallhorn, A;Wurm, FM
通讯作者：
Wurm, FM
影响因子：
5.1
作者：
Huang, YC;Simmons, C;Mooney, DJ
通讯作者：
Mooney, DJ