The interaction of ICP4 with cell/infected‐cell factors and its state of phosphorylation modulate differential recognition of leader sequences in herpes simplex virus DNA.
The interaction of ICP4 with cell/infected‐cell factors and its state of phosphorylation modulate differential recognition of leader sequences in herpes simplex virus DNA.
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ICP4 与细胞/感染细胞因子的相互作用及其磷酸化状态调节单纯疱疹病毒 DNA 中前导序列的差异识别。
作者：
A. Papavassiliou;K. Wilcox;S. Silverstein
单纯疱疹病毒（HSV）基因表达的调节需要功能性ICP 4的合成，ICP 4是一种磷蛋白，其结合病毒DNA中的几个特异性位点并反式作用以激活或抑制三种主要动力学类型的病毒基因的转录。ICP 4与α基因中的特定位点（首先被转录）的结合导致α基因表达的抑制。ICP 4还间接参与DNA-蛋白质复合物的形成，其序列存在于β和γ基因的启动子/调节和前导区中，这些基因在感染后期被顺序激活。在这里，我们证明了ICP 4的磷酸化程度有助于其差异参与与β和γ基因中存在的顺式作用元件形成复合物的能力。去磷酸化的ICP 4保留了其对α启动子中存在的高亲和力位点的结合特性，而只有磷酸化形式的蛋白质能够参与与模型β和γ序列的复合物形成。这些研究还揭示了细胞和感染细胞因子识别β和γ序列的要求。我们的数据表明，受感染细胞核内ICP 4的磷酸化状态和浓度决定了ICP 4与这些其他因子相互作用的程度。
Regulation of herpes simplex virus (HSV) gene expression requires the synthesis of functional ICP4, a phosphoprotein that binds to several specific sites in virus DNA and acts in trans either to activate or to repress transcription of the three major kinetic classes of virus genes. Binding of ICP4 to specific sites in alpha genes (which are the first to be transcribed) causes repression of alpha‐gene expression. ICP4 also indirectly participates in the formation of DNA‐protein complexes with sequences present in the promoter/regulatory and leader regions of the beta and gamma genes that are sequentially activated later in infection. Here we demonstrate that the extent of phosphorylation of ICP4 contributes to its ability to participate differentially in complex formation with cis‐acting elements present in beta and gamma genes. Dephosphorylated ICP4 retains its binding properties for the high affinity sites present in alpha promoters, whereas only phosphorylated forms of the protein are able to participate in complex formation with model beta and gamma sequences. These studies also reveal a requirement for cell and infected‐cell factors to recognize the beta and gamma sequences. Our data suggest that the state of phosphorylation and concentration of ICP4 within the nucleus of infected cells determine the extent to which ICP4 interacts with these other factors.
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DOI：
10.1073/pnas.83.13.4700
发表时间：
1986-07
影响因子：
11.1
作者：
T. Kristie;B. Roizman
通讯作者：
T. Kristie;B. Roizman
细胞和病毒蛋白与包含单纯疱疹病毒胸苷激酶基因的启动子/调节区和前导区的 DNA 序列的相互作用。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Papavassiliou,AG;Silverstein,SJ
通讯作者：
Silverstein,SJ
单纯疱疹病毒α蛋白ICP27可以抑制或增强病毒基因反式激活。
影响因子：
3.7
作者：
Su,L;Knipe,DM
通讯作者：
Knipe,DM
影响因子：
14.9
作者：
FABER, SW;WILCOX, KW
通讯作者：
WILCOX, KW
使用单纯疱疹病毒胸苷激酶基因序列表征核提取物中 DNA-蛋白质复合物的形成。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Papavassiliou,AG;Silverstein,SJ
通讯作者：
Silverstein,SJ