Competitive and substrate limited environments drive metabolic heterogeneity for comammox Nitrospira.
Competitive and substrate limited environments drive metabolic heterogeneity for comammox Nitrospira.
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竞争性和底物有限的环境驱动了Comammox硝基螺旋体的代谢异质性。
DOI：
10.1038/s43705-023-00288-8
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发表时间：
2023-08-29
期刊：
影响因子：
--
通讯作者：
Gonzalez-Cabaleiro, Rebeca
中科院分区：
文献类型：
--
作者：
Martinez-Rabert, Eloi;Smith, Cindy J.;Sloan, William T.;Gonzalez-Cabaleiro, Rebeca
硝化螺菌是一个多功能的属。虽然以前认为它只负责将亚硝酸盐转化为硝酸盐，但现在我们知道Nitrospira也可以将氨完全氧化为硝酸盐（comammox）。Comammox活性首先被报道为在氧气极其有限的环境中占主导地位，其中也发生厌氧氨氧化（anammox）。为了解释comammox的选择，我们开发了一个基于个体的模型，能够描述在动态氮和氧限制环境中组装的悬浮絮体中的硝化螺菌和厌氧氨氧化物的生长。所有已知的和假设的氮转化硝化螺菌被认为是：氨和亚硝酸盐氧化，comammox，硝酸盐还原氨氧化，和厌氧亚硝酸盐还原氨氧化。通过生物能量学分析，估计与每个活动相关的生长产量。描述生长所需的其他动力学参数根据所报告的文献值进行校准。我们的建模结果表明，即使是极低的氧气浓度（~1.0 µM）也允许厌氧氨氧化与硝化螺菌的成比例生长，与实验观察到的相似。强氧限制之后是氨和亚硝酸盐的限制，因为厌氧氨氧化，没有强大的竞争对手，能够比氮螺菌生长得更快，消耗环境中的氮。这些底物限制创造了一个极具竞争力的环境，这在硝化螺菌和厌氧氨氧化菌的群落组装中被证明是决定性的。此外，在所有测试条件下观察到Nitrospira的多种代谢活动，这反过来又解释了在具有较高氨可用性的有氧环境中观察到的瞬时亚硝酸盐积累。
Nitrospira has been revealed as a high versatile genus. Although previously considered only responsible for the conversion of nitrite to nitrate, now we know that Nitrospira can perform complete ammonia oxidation to nitrate too (comammox). Comammox activity was firstly reported as dominant in extremely limited oxygen environments, where anaerobic ammonia oxidation was also occurring (anammox). To explain the comammox selection, we developed an Individual-based Model able to describe Nitrospira and anammox growth in suspended flocs assembled in a dynamic nitrogen and oxygen-limiting environment. All known and hypothesized nitrogen transformations of Nitrospira were considered: ammonia and nitrite oxidation, comammox, nitrate-reducing ammonia oxidation, and anaerobic nitrite-reducing ammonia oxidation. Through bioenergetics analysis, the growth yield associated to each activity was estimated. The other kinetic parameters necessary to describe growth were calibrated according to the reported literature values. Our modeling results suggest that even extremely low oxygen concentrations (~1.0 µM) allow for a proportional growth of anammox versus Nitrospira similar to the one experimentally observed. The strong oxygen limitation was followed by a limitation of ammonia and nitrite, because anammox, without strong competitors, were able to grow faster than Nitrospira depleting the environment in nitrogen. These substrate limitations created an extremely competitive environment that proved to be decisive in the community assembly of Nitrospira and anammox. Additionally, a diversity of metabolic activities for Nitrospira was observed in all tested conditions, which in turn, explained the transient nitrite accumulation observed in aerobic environments with higher ammonia availability.
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影响因子：
16.6
作者：
Kits, K. Dimitri;Jung, Man-Young;Daims, Holger
通讯作者：
Daims, Holger
影响因子：
4.4
作者：
Nowka, Boris;Daims, Holger;Spieck, Eva
通讯作者：
Spieck, Eva
DOI：
10.1073/pnas.1405641111
发表时间：
2014-05-20
影响因子：
11.1
作者：
Mee, Michael T.;Collins, James J.;Wang, Harris H.
通讯作者：
Wang, Harris H.
影响因子：
12.8
作者：
Gonzalez-Cabaleiro, Rebeca;Curtis, Thomas Peter;Ofiteru, Irina Dana
通讯作者：
Ofiteru, Irina Dana
影响因子：
3.8
作者：
HUNIK, JH;BOS, CG;TRAMPER, J
通讯作者：
TRAMPER, J