Target sequences for cis-acting regulation within the dual promoter of the human c-myc gene
Target sequences for cis-acting regulation within the dual promoter of the human c-myc gene
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人c-myc基因双启动子内顺式作用调节的靶序列
DOI：
10.1128/mcb.7.4.1393-1400.1987
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发表时间：
1987
影响因子：
5.3
通讯作者：
G. Bornkamm
中科院分区：
文献类型：
--
作者：
M. Lipp;R. Schilling;S. Wiest;G. Laux;G. Bornkamm
构建了人c-myc启动子-前导区和细菌氯霉素乙酰转移酶（cat）基因的重组质粒。转染到不同的啮齿动物和人类细胞后，862碱基对（bp）的PvuII片段携带c-myc启动子和350 bp的非翻译前导赋予1/15至1/30的猴病毒40启动子介导的CAT活性。PvuII片段上游的额外序列的存在对c-myc启动子活性具有总体负面影响，可通过用少量转染的质粒DNA进行滴定分析来检测。对c-myc启动子-前导区中的许多缺失构建体的分析以及S1作图实验表明，高CAT活性在很大程度上取决于第二启动子的存在。通过将c-myc-cat构建体与携带c-myc启动子基因座的不同部分的质粒共转染，鉴定了正作用细胞因子的靶标。两个正调控元件内的862 bp的PvuII片段映射。一个位于第一个帽位点上游-101至-349 bp的248-bp PvuII-SmaI片段内，另一个位于第一个外显子的142-pb XhoI-NaeI片段内，包括相对于第二个帽位点的位置-95至+47。我们的结论是，人类c-myc基因的双启动子代表了一个强大的真核启动子的正，负作用的细胞转录因子的合作调节。
Recombinant plasmids of the human c-myc promoter-leader region and the bacterial chloramphenicol acetyltransferase (cat) gene were constructed. After transfection into different rodent and human cells, the 862-base-pair (bp) PvuII fragment carrying both c-myc promoters and 350 bp of the untranslated leader conferred 1/15 to 1/30 of the CAT activity mediated by the simian virus 40 promoter. The presence of additional sequences upstream of the PvuII fragment had an overall negative effect on c-myc promoter activity detectable by titration analysis with small amounts of transfected plasmid DNA. The analysis of numerous deletion constructs in the c-myc promoter-leader region as well as S1 mapping experiments demonstrated that the high CAT activity depended largely on the presence of the second promoter. By cotransfection of c-myc-cat constructs with plasmids carrying different parts of the c-myc promoter locus, targets for positively acting cellular factors were identified. Two positive regulatory elements were mapped within the 862-bp PvuII fragment. One was localized within the 248-bp PvuII-SmaI fragment -101 to -349 bp upstream of the first cap site and the other within the 142-pb XhoI-NaeI fragment of the first exon, comprising positions -95 to +47 relative to the second cap site. We conclude that the dual promotor of the human c-myc gene represents a strong eucaryotic promotor regulated by cooperation of positively and negatively acting cellular transcription factors.
DOI：
10.1073/pnas.80.20.6307
发表时间：
1983-10
影响因子：
11.1
作者：
R. Watt;K. Nishikura;J. Sorrentino;A. Ar-rushdi;C. Croce;G. Rovera
通讯作者：
R. Watt;K. Nishikura;J. Sorrentino;A. Ar-rushdi;C. Croce;G. Rovera
禽成髓细胞瘤病毒的遗传结构，从转化的成髓细胞中作为有缺陷的病毒颗粒释放。
DOI：
10.1073/pnas.77.9.5120
发表时间：
1980
影响因子：
11.1
作者：
Duesberg,PH;Bister,K;Moscovici,C
通讯作者：
Moscovici,C