Overcoming the fundamental limitation of frequency-domain fluorescence lifetime imaging microscopy spatial resolution
Overcoming the fundamental limitation of frequency-domain fluorescence lifetime imaging microscopy spatial resolution
复制标题
克服频域荧光寿命成像显微镜空间分辨率的基本限制
DOI：
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
S. Howard
中科院分区：
文献类型：
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作者：
Xiaotong Yuan;Varun Mannam;Yide Zhang;S. Howard
我们提出并证明了频域（FD）荧光寿命成像显微镜（FLIM）的空间分辨率的第一个分析模型，解释了它是如何从根本上不同于传统荧光显微镜的常见分辨率限制。该模型还观察到调频（FM）捕获效应，这导致FLIM测量失真。提出了一种基于超分辨径向起伏（SRRF）的超分辨FLIM方法。在这种方法中，我们分别处理强度和寿命，以生成超分辨率FLIM合成图像。该方法的能力进行了验证，在固定的细胞样本的数值和实验。
We propose and demonstrate the first analytical model of the spatial resolution of frequency-domain (FD) fluorescence lifetime imaging microscopy (FLIM) that explains how it is fundamentally different with the common resolution limit of the conventional fluorescence microscopy. Frequency modulation (FM) capture effect is also observed by the model, which results in distorted FLIM measurements. A super-resolution FLIM approach based on a localization-based technique, super-resolution radial fluctuations (SRRF), is presented. In this approach, we separately process the intensity and lifetime to generate a super-resolution FLIM composite image. The capability of the approach is validated both numerically and experimentally in fixed cells sample.
DOI：
10.2174/2211555203666141117221111
发表时间：
2014
期刊：
Current molecular imaging
影响因子：
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作者：
Rajoria S;Zhao L;Intes X;Barroso M
通讯作者：
Barroso M