Unpicking the Secrets of African Swine Fever Viral Replication Sites.
Unpicking the Secrets of African Swine Fever Viral Replication Sites.
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DOI：
10.3390/v13010077
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发表时间：
2021-01-08
期刊：
影响因子：
--
通讯作者：
Hawes PC
中科院分区：
文献类型：
--
作者：
Aicher SM;Monaghan P;Netherton CL;Hawes PC
非洲猪瘟病毒(ASFV)是一种高度传染性的病原体，可引起家猪和野猪的致死性出血热。这种大型双链DNA病毒在被称为病毒工厂的核周细胞质复制位置进行复制。这些工厂是复杂的、多维的结构。在这里，我们使用超分辨率和电子断层扫描对工厂的蛋白质和膜隔间进行了研究。Click IT化学结合受激发射耗尽(STED)显微镜显示了新合成的病毒蛋白的网状网络，包括结构蛋白p54和p34，以前在共聚焦显微镜下看到的是一个多形带。电子显微镜和断层扫描证实，该网络是膜组装中间体的积累，具有多种形式。在工厂形成的早期时间点，这些中间体以小的单个膜碎片的形式出现，它们似乎在不断地生长并连接在一起，朝着新的二十面体病毒粒子发展。目前尚不清楚这些膜是如何形成的，以及在病毒形态发生期间它们是如何运输到工厂的。
African swine fever virus (ASFV) is a highly contagious pathogen which causes a lethal haemorrhagic fever in domestic pigs and wild boar. The large, double-stranded DNA virus replicates in perinuclear cytoplasmic replication sites known as viral factories. These factories are complex, multi-dimensional structures. Here we investigated the protein and membrane compartments of the factory using super-resolution and electron tomography. Click IT chemistry in combination with stimulated emission depletion (STED) microscopy revealed a reticular network of newly synthesized viral proteins, including the structural proteins p54 and p34, previously seen as a pleomorphic ribbon by confocal microscopy. Electron microscopy and tomography confirmed that this network is an accumulation of membrane assembly intermediates which take several forms. At early time points in the factory formation, these intermediates present as small, individual membrane fragments which appear to grow and link together, in a continuous progression towards new, icosahedral virions. It remains unknown how these membranes form and how they traffic to the factory during virus morphogenesis.
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影响因子：
6.7
作者：
Castelló A;Quintas A;Sánchez EG;Sabina P;Nogal M;Carrasco L;Revilla Y
通讯作者：
Revilla Y
DOI：
10.1083/jcb.153.3.449
发表时间：
2001-04-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Heath CM;Windsor M;Wileman T
通讯作者：
Wileman T
影响因子：
3
作者：
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通讯作者：
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影响因子：
5.4
作者：
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通讯作者：
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影响因子：
3.4
作者：
Krijnse Locker J;Chlanda P;Sachsenheimer T;Brügger B
通讯作者：
Brügger B