Aggresomes resemble sites specialized for virus assembly.
Aggresomes resemble sites specialized for virus assembly.
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DOI：
10.1083/jcb.153.3.449
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发表时间：
2001-04-30
期刊：
影响因子：
--
通讯作者：
Wileman T
中科院分区：
文献类型：
--
作者：
Heath CM;Windsor M;Wileman T
大型细胞质DNA病毒，如痘病毒、虹膜病毒和非洲猪瘟病毒（ASFV）聚集在称为病毒工厂的离散核周围病灶中。工厂排除宿主蛋白，表明它们是由病毒诱导的新型亚细胞结构。新的核周结构，称为聚合体，也由细胞对错误折叠蛋白的反应形成（Johnston, j.a.， C.L. Ward和R.R. Kopito. 1998）。[j] .细胞生物学杂志。143:1883-1898；García-Mata， R.， Z. Bebök， E.J. Sorscher， E.S. Sztul. 1999。[j] .细胞生物学杂志。146:1239-1254。在这项研究中，我们研究了聚合体和病毒工厂是否相关的结构。将聚合体与ASFV产生的病毒工厂进行了比较。聚集体和病毒工厂位于微管组织中心附近，需要一个完整的微管网络进行组装。这两种结构都引起了中间细丝的重排和维门蛋白在特定的笼子中坍塌，并且都招募了线粒体和细胞伴侣。鉴于ASFV工厂类似于聚合体，细胞质DNA病毒可能利用最初设计用于降低错误折叠蛋白毒性的细胞反应，将结构蛋白集中在病毒组装位点。
The large cytoplasmic DNA viruses such as poxviruses, iridoviruses, and African swine fever virus (ASFV) assemble in discrete perinuclear foci called viral factories. Factories exclude host proteins, suggesting that they are novel subcellular structures induced by viruses. Novel perinuclear structures, called aggresomes are also formed by cells in response to misfolded protein (Johnston, J.A., C.L. Ward, and R.R. Kopito. 1998. J. Cell Biol. 143:1883–1898; García-Mata, R., Z. Bebök, E.J. Sorscher, and E.S. Sztul. 1999. J. Cell Biol. 146:1239–1254). In this study, we have investigated whether aggresomes and viral factories are related structures. Aggresomes were compared with viral factories produced by ASFV. Aggresomes and viral factories were located close to the microtubule organizing center and required an intact microtubular network for assembly. Both structures caused rearrangement of intermediate filaments and the collapse of vimentin into characteristic cages, and both recruited mitochondria and cellular chaperones. Given that ASFV factories resemble aggresomes, it is possible that a cellular response originally designed to reduce the toxicity of misfolded proteins is exploited by cytoplasmic DNA viruses to concentrate structural proteins at virus assembly sites.
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DOI：
10.1083/jcb.143.7.1883
发表时间：
1998-12-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Johnston JA;Ward CL;Kopito RR
通讯作者：
Kopito RR
影响因子：
3.8
作者：
Sanderson, CM;Hollinshead, M;Smith, GL
通讯作者：
Smith, GL
影响因子：
2.7
作者：
MOURANUNES, JF;VIGARIO, JD;TERRINHA, AM
通讯作者：
TERRINHA, AM
影响因子：
3.7
作者：
SHARPE, AH;CHEN, LB;FIELDS, BN
通讯作者：
FIELDS, BN
影响因子：
5.4
作者：
Cobbold, C;Wileman, T
通讯作者：
Wileman, T