Phenotypical characterization of regulatory T cells in acute Zika infection.
Phenotypical characterization of regulatory T cells in acute Zika infection.
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DOI：
10.1016/j.cyto.2021.155651
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发表时间：
2021-10
期刊：
影响因子：
3.8
通讯作者：
Keesen TSL
中科院分区：
文献类型：
--
作者：
Guerra-Gomes IC;Gois BM;Peixoto RF;Palmeira PHS;Dias CNS;Csordas BG;Araújo JMG;Veras RC;de Medeiros IA;de Azevedo FLAA;Boyton RJ;Altmann DM;Keesen TSL
寨卡病毒(ZIKV)、登革病毒(DENV)、基孔肯雅病毒(CHIKV)和黄热病病毒(YFV)是美洲流行的虫媒病毒。每一种感染都与相关疾病免疫病理学的发展有关。免疫病理过程是效应器和调节性免疫机制之间相互平衡影响的结果。在这种情况下，调节性T细胞(Treg)是调节免疫反应的关键，因此在组织损伤控制中也是关键。然而，到目前为止，人类急性感染ZIKV期间Treg的表型和机制还没有得到充分的研究。这项工作的主要目的是表征Tregs及其与细胞因子产生相关的免疫学特征，以及能够控制急性寨卡病毒感染患者恶化炎症特征的分子。通过对感染患者的全血分析，采用流式细胞术对急性寨卡病毒感染过程中循环的Tregs的体外表型特征进行了研究。我们发现，尽管在感染组和健康对照组之间，Treg的绝对频率没有差异。而促炎性细胞因子如干扰素-γ和LAP在急性期表达上调。此外，急性ZIKV患者CD39/CD73、穿孔素/颗粒酶B、PD-1和CTLA-4水平升高，所有这些标志物都参与了Tregs试图控制强烈炎症反应的机制。因此，数据表明Tregs在炎症性ZIKV感染反应中可能起到了作用。
Zika virus (ZIKV), alongside Dengue virus (DENV), Chikungunya virus (CHIKV), and Yellow Fever Virus (YFV) are prevalent arboviruses in the Americas. Each of these infections is associated with the development of associated disease immunopathology. Immunopathological processes are an outcome of counter-balancing impacts between effector and regulatory immune mechanisms. In this context, regulatory T cells (Tregs) are key in modulating the immune response and, therefore, in tissue damage control. However, to date, Treg phenotypes and mechanisms during acute infection of the ZIKV in humans have not been fully investigated. The main aim of this work was to characterize Tregs and their immunological profile related to cytokine production and molecules that are capable of controlling the exacerbated inflammatory profile in acute Zika infected patients. Using whole blood analyses of infected patients, an ex vivo phenotypical characterization of Tregs, circulating during acute Zika virus infection, was conducted by flow cytometry. We found that though there are no differences in absolute Treg frequency between infected and healthy control groups. However, pro-inflammatory cytokine up-regulation such as IFN-γ and LAP was observed in the acute disease. Furthermore, acute ZIKV patients expressed increased levels of CD39/CD73, perforin/granzyme B, PD-1, and CTLA-4, all markers involved in mechanisms used by Tregs to attempt to control strong inflammatory responses. Thus, the data indicates a potential contribution of Tregs during the inflammatory ZIKV infection response.
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影响因子：
6.7
作者：
Manangeeswaran M;Ireland DD;Verthelyi D
通讯作者：
Verthelyi D
DOI：
10.1093/cid/cix732
发表时间：
2018-01-06
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Lai L;Rouphael N;Xu Y;Natrajan MS;Beck A;Hart M;Feldhammer M;Feldpausch A;Hill C;Wu H;Fairley JK;Lankford-Turner P;Kasher N;Rago P;Hu YJ;Edupuganti S;Patel SM;Murray KO;Mulligan MJ;Emory Zika Patient Study Team
通讯作者：
Emory Zika Patient Study Team
影响因子：
5.4
作者：
Tappe D;Pérez-Girón JV;Zammarchi L;Rissland J;Ferreira DF;Jaenisch T;Gómez-Medina S;Günther S;Bartoloni A;Muñoz-Fontela C;Schmidt-Chanasit J
通讯作者：
Schmidt-Chanasit J
DOI：
10.1093/infdis/jiy225
发表时间：
2018-07-24
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Lum FM;Lye DCB;Tan JJL;Lee B;Chia PY;Chua TK;Amrun SN;Kam YW;Yee WX;Ling WP;Lim VWX;Pang VJX;Lee LK;Mok EWH;Chong CY;Leo YS;Ng LFP
通讯作者：
Ng LFP
影响因子：
4.6
作者：
Cimini, Eleonora;Castilletti, Concetta;Agrati, Chiara
通讯作者：
Agrati, Chiara