Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation.
Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation.
复制标题
DOI：
10.1021/acssynbio.1c00311
复制
发表时间：
2022-01-21
影响因子：
4.7
通讯作者：
Liu LN
中科院分区：
文献类型：
--
作者：
Chen T;Fang Y;Jiang Q;Dykes GF;Lin Y;Price GD;Long BM;Liu LN
碳氧体是原核生物细胞器的一个通用范例，是一种蛋白质自组装微区室，在所有蓝藻和一些化学自养生物的碳固定中起着重要作用。羧基体使用多面体蛋白质壳包封中心CO2固定酶，核酮糖-1，5-二磷酸羧化酶/加氧酶（Rubisco），所述多面体蛋白质壳选择性地渗透特定代谢物以有利于Rubisco羧化。在异源生物中，重新利用羧基体来促进碳固定具有巨大的兴趣。在此，我们通过在大肠杆菌中与α-羧化酶体共表达Rubisco活化酶组分CbbQ和CbbO来开发α-羧化酶体的设计和工程。我们的研究结果表明，CbbQ和CbbO可以组装到重构的α-羧基体作为内在成分。将CbbQ和CbbO两者并入羧基体内促进Rubisco的活化并增强重组羧基体的CO2固定活性。我们还表明，这些羧基体的结构组成可以在不同的表达系统中进行修改，代表的可塑性的羧基体架构。在翻译方面，我们的研究为在各种生物技术应用中工程化和调节羧基体提供了策略。
The carboxysome is a versatile paradigm of prokaryotic organelles and is a proteinaceous self-assembling microcompartment that plays essential roles in carbon fixation in all cyanobacteria and some chemoautotrophs. The carboxysome encapsulates the central CO2-fixing enzyme, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), using a polyhedral protein shell that is selectively permeable to specific metabolites in favor of Rubisco carboxylation. There is tremendous interest in repurposing carboxysomes to boost carbon fixation in heterologous organisms. Here, we develop the design and engineering of α-carboxysomes by coexpressing the Rubisco activase components CbbQ and CbbO with α-carboxysomes in Escherichia coli. Our results show that CbbQ and CbbO could assemble into the reconstituted α-carboxysome as intrinsic components. Incorporation of both CbbQ and CbbO within the carboxysome promotes activation of Rubisco and enhances the CO2-fixation activities of recombinant carboxysomes. We also show that the structural composition of these carboxysomes could be modified in different expression systems, representing the plasticity of the carboxysome architecture. In translational terms, our study informs strategies for engineering and modulating carboxysomes in diverse biotechnological applications.
登录
查看更多内容
影响因子：
7.5
作者：
Cornejo, Elias;Abreu, Nicole;Komeili, Arash
通讯作者：
Komeili, Arash
影响因子：
3.7
作者：
Engler, Carola;Kandzia, Romy;Marillonnet, Sylvestre
通讯作者：
Marillonnet, Sylvestre
影响因子：
7.7
作者：
Flamholz AI;Dugan E;Blikstad C;Gleizer S;Ben-Nissan R;Amram S;Antonovsky N;Ravishankar S;Noor E;Bar-Even A;Milo R;Savage DF
通讯作者：
Savage DF
影响因子：
16.6
作者：
Hagen, Andrew;Sutter, Markus;Kerfeld, Cheryl A.
通讯作者：
Kerfeld, Cheryl A.
影响因子：
2.9
作者：
BADGER, MR;LORIMER, GH
通讯作者：
LORIMER, GH