Cannabinoids Regulate the Diameter of Pericyte-Containing Retinal Capillaries in Rats
Cannabinoids Regulate the Diameter of Pericyte-Containing Retinal Capillaries in Rats
复制标题
大麻素调节大鼠含有周细胞的视网膜毛细血管的直径
DOI：
10.1159/000484193
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发表时间：
2017-10
影响因子：
--
通讯作者：
Chunhui Jiang
中科院分区：
文献类型：
--
作者：
Yuan Zong;Xujiao Zhou;Jingyi Cheng;Jian Yu;Jihong Wu;Chunhui Jiang
背景/目标：大麻素是血管活性物质，在供应外周组织和中枢神经系统的血管中充当动脉张力的关键调节剂。因此，我们研究了大麻素对视网膜毛细血管和周细胞的影响。方法：使用离体整体大鼠视网膜模型测定大麻素对毛细血管直径的影响。通过免疫印迹、定量PCR和免疫组化等方法探讨其机制。结果如下：内源性大麻素2-花生四烯酰甘油和大麻素和外源性大麻素（R-（+）-WIN 55212 -2）以浓度依赖性方式（1 µM至0.1 mM）扩张去甲肾上腺素预收缩的毛细血管。血管舒张的程度与周细胞宽度的变化呈正相关。R-（+）-WIN 55212 -2对血管舒张和周细胞宽度的作用被大麻素受体1型（CB 1）拮抗剂AM 251或利莫那班（SR 141716 A）、一氧化氮合酶抑制剂l-NAME和鸟苷酸环化酶抑制剂ODQ抑制。他们也废除了内皮细胞的去除，但不是由大麻素受体-2拮抗剂SR 144528，内皮大麻素受体拮抗剂O-1918，或环氧合酶抑制剂吲哚美辛。结论：外源性大麻素R-（+）-WIN 55212 -2对含周细胞的大鼠视网膜毛细血管有舒张作用。R-（+）-WIN 55212 -2的这种作用依赖于CB 1和一氧化氮-环磷酸鸟苷途径，并且需要完整的内皮。
Background/Aims: Cannabinoids are vasoactive substances that act as key regulators of arterial tone in the blood vessels supplying peripheral tissues and the central nervous system. We therefore investigated the effect of cannabinoids on retinal capillaries and pericytes. Methods: The effects of cannabinoids on capillary diameters were determined using an ex vivo whole-mount rat retinal model. Western blotting, quantitative PCR, and immunohistochemistry were performed to explore the underlying mechanism. Results: Endogenous cannabinoid 2-arachidonoylglycerol and anandamide and exogenous cannabinoid (R-(+)-WIN55212-2) dilated the noradrenaline-precontracted capillaries in a concentration-dependent manner (1 µM to 0.1 mM). The extent of vasorelaxation was positively correlated with changes in pericyte width. The effects of R-(+)-WIN55212-2 on vasorelaxation and pericyte width were inhibited by a cannabinoid receptor type-1 (CB1) antagonist, AM251 or rimonabant (SR141716A), the nitric oxide synthase inhibitor l-NAME, and the guanylate cyclase inhibitor ODQ. They were also abolished by the removal of the endothelium, but not by the cannabinoid receptor-2 antagonist SR144528, the endothelial cannabinoid receptor antagonist O-1918, or the cyclooxygenase inhibitor indomethacin. Conclusion: The exogenous cannabinoid R-(+)-WIN55212-2 promotes the vasorelaxation of pericyte-containing rat retinal capillaries. This effect of R-(+)-WIN55212-2 is dependent on CB1 and the nitric oxide–cyclic guanosine monophosphate pathway, and requires an intact endothelium.
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影响因子：
3.1
作者：
Kokona D;Georgiou PC;Kounenidakis M;Kiagiadaki F;Thermos K
通讯作者：
Thermos K
影响因子：
--
作者：
Jenkin, Kayte A.;McAinch, Andrew J.;Hryciw, Deanne H.
通讯作者：
Hryciw, Deanne H.
影响因子：
4.4
作者：
E. Su;M. Kelly;S. Cringle;Dao-Yi Yu
通讯作者：
E. Su;M. Kelly;S. Cringle;Dao-Yi Yu
影响因子：
5.5
作者：
Matsushita, Kenji;Puro, Donald G.
通讯作者：
Puro, Donald G.
影响因子：
4.8
作者：
Maccarrone, M;Bari, M;Finazzi-Agrò, A
通讯作者：
Finazzi-Agrò, A