Rapid electrical impedance detection of sickle cell vaso-occlusion in microfluidic device
Rapid electrical impedance detection of sickle cell vaso-occlusion in microfluidic device
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微流控装置中镰状细胞血管闭塞的快速电阻抗检测
DOI：
10.1007/s10544-023-00663-1
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发表时间：
2023
影响因子：
2.8
通讯作者：
Alvarez, Ofelia
中科院分区：
文献类型：
--
作者：
Qiang, Yuhao;Dieujuste, Darryl;Liu, Jia;Alvarez, Ofelia
镰状细胞病的特征是疼痛的血管闭塞危象，其中变形不良的镰状细胞在复杂的血管阻塞过程中起重要作用。现有的技术主要是基于光学显微镜和视频处理的镰状血流在常氧条件下，用于测量血管闭塞的一小部分致密的镰状细胞的固有刚性，但不是血管闭塞的刚性，镰状细胞由于脱氧。因此，这些技术不适用于快速、即时检测。在这里，我们将电阻抗传感和聚二甲基硅氧烷微血管模拟物与受控的氧气水平集成到单个微流体芯片中，用于在1分钟内定量刚性镰状细胞的血管闭塞。电阻抗测量提供了一种无标记的，实时检测不同的镰状细胞流动行为，包括稳定的流动，血管闭塞，并响应于脱氧-复氧过程的流动恢复，通过显微镜视频验证。比较了阻抗信号的真实的部分和虚部对自然镰状细胞血液和模拟物中四种电频率（10、50、100和500 kHz）下的血流条件的灵敏度。结果表明，随着电频率的增加，传感器检测血管阻塞的灵敏度降低，而更高的频率更适合于测量稳态流动行为。使用镰状细胞模拟物、化学交联的正常红细胞的附加测试在检测血管闭塞方面显示出与脱氧下的镰状细胞血管闭塞相同的高灵敏度。这项工作使即时检测的潜力，在快速，准确地检测稳定的流动和镰状细胞血管闭塞微升体积的血液标本。定量的镰状细胞的流变性在缺氧反应，不仅可以提供有用的指示细胞镰状化的动力学，但也改变了血流动力学观察到的微循环水平。
Sickle cell disease is characterized by painful vaso-occlusive crises, in which poorly deformable sickle cells play an important role in the complex vascular obstruction process. Existing techniques are mainly based on optical microscopy and video processing of sickle blood flow under normoxic condition, for measuring vaso-occlusion by a small fraction of dense sickle cells of intrinsic rigidity but not the vaso-occlusion by the rigid, sickled cells due to deoxygenation. Thus, these techniques are not suitable for rapid, point-of-care testing. Here, we integrate electrical impedance sensing and Polydimethylsiloxane-microvascular mimics with controlled oxygen level into a single microfluidic chip, for quantification of vaso-occlusion by rigid, sickled cells within 1 min. Electrical impedance measurements provided a label-free, real-time detection of different sickle cell flow behaviors, including steady flow, vaso-occlusion, and flow recovery in response to the deoxygenation-reoxygenation process that are validated by microscopic videos. Sensitivity of the real part and imaginary part of the impedance signals to the blood flow conditions in both natural sickle cell blood and simulants at four electrical frequencies (10, 50, 100, and 500 kHz) are compared. The results show that the sensitivity of the sensor in detection of vaso-occlusion decreases as electrical frequency increases, while the higher frequencies are preferable in measurement of steady flow behavior. Additional testing using sickle cell simulants, chemically crosslinked normal red blood cells, shows same high sensitivity in detection of vaso-occlusion as sickle cell vaso-occlusion under deoxygenation. This work enables point-of-care testing potentials in rapid, accurate detection of steady flow and sickle cell vaso-occlusion from microliter volume blood specimens. Quantification of sickle cell rheology in response to hypoxia, may provide useful indications for not only the kinetics of cell sickling, but also the altered hemodynamics as obseved at the microcirculatory level.
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影响因子：
15.9
作者：
Tsai, Michelle;Kita, Ashley;Lam, Wilbur A.
通讯作者：
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氧张力、温度和血红蛋白浓度对镰状红细胞流变特性的影响。
影响因子：
20.3
作者：
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通讯作者：
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作者：
Man Y;Maji D;An R;Ahuja SP;Little JA;Suster MA;Mohseni P;Gurkan UA
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Gurkan UA
DOI：
10.1073/pnas.1424111112
发表时间：
2015-02-03
影响因子：
11.1
作者：
Du, E.;Diez-Silva, Monica;Suresh, Subra
通讯作者：
Suresh, Subra
镰状细胞病的血管闭塞：当前概念和未解答的问题。
影响因子：
20.3
作者：
R. Francis;Cage S. Johnson
通讯作者：
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