A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.
A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.
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DOI：
10.1371/journal.pone.0022261
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Saito MK
中科院分区：
文献类型：
--
作者：
Niwa A;Heike T;Umeda K;Oshima K;Kato I;Sakai H;Suemori H;Nakahata T;Saito MK
阐明人胚胎干细胞（ES）和诱导多能干细胞（iPS）的体外分化对于理解体内正常和病理造血发育都是重要的。为此，一个强大的和简单的造血分化系统，可以忠实地跟踪体内造血是必要的。在这项研究中，我们建立了一种新的无血清单层培养，可以跟踪从ES/iPS细胞通过中胚层祖细胞到功能性定形血细胞的体内造血途径。外源性细胞因子混合物通过原条细胞诱导造血中胚层祖细胞。然后，这些祖细胞根据存在的造血细胞因子分化成各种细胞谱系。此外，单细胞沉积实验显示，在我们的培养中诱导了常见的双能血血管生成祖细胞。我们的系统提供了一个新的，强大的，简单的方法，调查中胚层和造血分化的机制。
Elucidating the in vitro differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells is important for understanding both normal and pathological hematopoietic development in vivo. For this purpose, a robust and simple hematopoietic differentiation system that can faithfully trace in vivo hematopoiesis is necessary. In this study, we established a novel serum-free monolayer culture that can trace the in vivo hematopoietic pathway from ES/iPS cells to functional definitive blood cells via mesodermal progenitors. Stepwise tuning of exogenous cytokine cocktails induced the hematopoietic mesodermal progenitors via primitive streak cells. These progenitors were then differentiated into various cell lineages depending on the hematopoietic cytokines present. Moreover, single cell deposition assay revealed that common bipotential hemoangiogenic progenitors were induced in our culture. Our system provides a new, robust, and simple method for investigating the mechanisms of mesodermal and hematopoietic differentiation.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
DOI：
10.1073/pnas.0802220105
发表时间：
2008-09-02
影响因子：
11.1
作者：
Ma, Feng;Ebihara, Yasuhiro;Tsuji, Kohichiro
通讯作者：
Tsuji, Kohichiro
影响因子：
5.2
作者：
Ji, Junfeng;Vijayaragavan, Kausalia;Bhatia, Mickie
通讯作者：
Bhatia, Mickie
影响因子：
2.1
作者：
Fujimi, Akihito;Matsunaga, Takuya;Niitsu, Yoshiro
通讯作者：
Niitsu, Yoshiro
影响因子：
4.6
作者：
Martin, R;Lahlil, R;Hoang, T
通讯作者：
Hoang, T