Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor.
Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor.
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DOI：
10.1093/nar/gks1203
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发表时间：
2013-02-01
影响因子：
14.9
通讯作者：
Martín JF
中科院分区：
文献类型：
--
作者：
Sola-Landa A;Rodríguez-García A;Amin R;Wohlleben W;Martín JF
调控网络的相互作用是细菌系统生物学研究的一个重要课题。链霉菌代谢的磷酸盐调控是由磷-磷双组分系统介导的。同样，不同氮源的利用由调节因子GlnR控制。转录和生化分析表明，编码谷氨酰胺合成酶的glnA、glnR和其他氮代谢基因受Phop控制。DNA结合实验表明，Phop与其他氮调控基因(SCO0255、SCO01863和尿素)结合。以GlnA启动子为模型，我们观察到Phop和GlnR竞争结合到同一个启动子区域，显示出GlnR具有更高的亲和力。利用总共14个GlnR结合位点(50个直接重复单位)，我们建立了两个基于信息的模型，将GlnR box描述为由两个11-nT的直接重复组成，每个重复与pho box有明显的差异。与不同突变的glnA启动子序列的DNA结合研究表明，GlnR识别的序列在编码链中，而Phop识别的序列在非编码链中重叠。在amtB启动子中，Phop和GlnR盒并不完全重叠，两种蛋白同时结合。PHOP调控氮代谢基因有助于平衡细胞内的P/N平衡。
Interaction of regulatory networks is a subject of great interest in systems biology of bacteria. Phosphate control of metabolism in Streptomyces is mediated by the two-component system PhoR–PhoP. Similarly, the utilization of different nitrogen sources is controlled by the regulator GlnR. Transcriptomic and biochemical analysis revealed that glnA (encoding a glutamine synthetase), glnR and other nitrogen metabolism genes are under PhoP control. DNA-binding experiments showed that PhoP binds to other nitrogen-regulated genes (SCO0255, SCO01863 and ureA). Using the glnA promoter as model, we observed that PhoP and GlnR compete for binding to the same promoter region, showing GlnR a higher affinity. Using a total of 14 GlnR-binding sites (50 direct repeat units) we established two information-based models that describe the GlnR box as consisting of two 11-nt direct repeats each with clear differences to PHO box. DNA-binding studies with different mutant sequences of glnA promoter revealed that the sequence recognized by GlnR is found in the coding strand whereas that recognized by PhoP is overlapping in the non-coding strand. In amtB promoter PhoP and GlnR boxes are not totally overlapping and both proteins bind simultaneously. PhoP control of nitrogen metabolism genes helps to balance the cellular P/N equilibrium.
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DOI：
10.1099/00221287-145-9-2313
发表时间：
1999-09-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Fink, D;Falke, D;Engels, A
通讯作者：
Engels, A
影响因子：
3.6
作者：
Hesketh, A;Fink, D;Engels, A
通讯作者：
Engels, A
影响因子：
5.7
作者：
Blanco, AG;Sola, M;Coll, M
通讯作者：
Coll, M
影响因子：
2.8
作者：
Apel, Alexander K.;Sola-Landa, Alberto;Martin, Juan F.
通讯作者：
Martin, Juan F.
影响因子：
2
作者：
Schneider, TD
通讯作者：
Schneider, TD