Laboratory-scale method for enzymatic saccharification of lignocellulosic biomass at high-solids loadings.
Laboratory-scale method for enzymatic saccharification of lignocellulosic biomass at high-solids loadings.
复制标题
DOI：
10.1186/1754-6834-2-28
复制
发表时间：
2009-11-04
影响因子：
6.3
通讯作者：
Stickel JJ
中科院分区：
文献类型：
--
作者：
Roche CM;Dibble CJ;Stickel JJ
在工艺相关条件下筛选新的木质纤维素生物质预处理和先进的酶系统是开发经济可行的木质纤维素乙醇的关键因素。摇瓶是通常用于筛选纤维素生物质的酶糖化的反应容器，当摇动不辅以手动混合时，摇瓶不能在高固体浓度下提供充分的混合。我们将滚瓶反应器（RBRs）确定为实验室规模的反应容器，其可以在高固体生物质负载下为酶促糖化提供充分的混合，而无需任何额外的手动混合。使用RBRs，我们开发了一种方法，用于在工艺相关条件下筛选预处理的生物质和酶系统。RBRs显示在125 mL和2 L之间可扩展。五个不同严重程度的生物质预处理和两种酶制剂的酶糖化结果表明，该系统将工作良好的各种生物质底物和酶系统。间歇混合制度的研究表明，在高固体负荷的酶糖化的传质限制是显着的，但可以减轻与一个相对较低的量的混合输入。有效的初始混合以促进良好的酶分布和持续的但不一定是连续的混合是必要的，以促进高生物质转化率。实验室规模的RBR系统的简单性和鲁棒性，结合其适应众多反应容器的能力，将有助于在高固体负载下筛选新的生物质预处理和先进的酶系统。
Screening new lignocellulosic biomass pretreatments and advanced enzyme systems at process relevant conditions is a key factor in the development of economically viable lignocellulosic ethanol. Shake flasks, the reaction vessel commonly used for screening enzymatic saccharifications of cellulosic biomass, do not provide adequate mixing at high-solids concentrations when shaking is not supplemented with hand mixing. We identified roller bottle reactors (RBRs) as laboratory-scale reaction vessels that can provide adequate mixing for enzymatic saccharifications at high-solids biomass loadings without any additional hand mixing. Using the RBRs, we developed a method for screening both pretreated biomass and enzyme systems at process-relevant conditions. RBRs were shown to be scalable between 125 mL and 2 L. Results from enzymatic saccharifications of five biomass pretreatments of different severities and two enzyme preparations suggest that this system will work well for a variety of biomass substrates and enzyme systems. A study of intermittent mixing regimes suggests that mass transfer limitations of enzymatic saccharifications at high-solids loadings are significant but can be mitigated with a relatively low amount of mixing input. Effective initial mixing to promote good enzyme distribution and continued, but not necessarily continuous, mixing is necessary in order to facilitate high biomass conversion rates. The simplicity and robustness of the bench-scale RBR system, combined with its ability to accommodate numerous reaction vessels, will be useful in screening new biomass pretreatments and advanced enzyme systems at high-solids loadings.
登录
查看更多内容
影响因子：
3.8
作者：
Jorgensen, Henning;Vibe-Pedersen, Jakob;Felby, Claus
通讯作者：
Felby, Claus
影响因子：
3.8
作者：
Roche, Christine M.;Dibble, Clare J.;Liberatore, Matthew W.
通讯作者：
Liberatore, Matthew W.
影响因子：
11.4
作者：
Selig, Michael J.;Knoshaug, Eric P.;Decker, Stephen R.
通讯作者：
Decker, Stephen R.
影响因子：
3.8
作者：
Yang, B;Wyman, CE
通讯作者：
Wyman, CE
影响因子：
3.8
作者：
Jeoh, Tina;Ishizawa, Claudia I.;Johnson, David K.
通讯作者：
Johnson, David K.