The clinical significance of the CD163+ and CD68+ macrophages in patients with hepatocellular carcinoma.
The clinical significance of the CD163+ and CD68+ macrophages in patients with hepatocellular carcinoma.
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DOI：
10.1371/journal.pone.0059771
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sun HC
中科院分区：
文献类型：
--
作者：
Kong LQ;Zhu XD;Xu HX;Zhang JB;Lu L;Wang WQ;Zhang QB;Wu WZ;Wang L;Fan J;Tang ZY;Sun HC
我们以前的研究发现，肿瘤周围的CD68+巨噬细胞的丰度与肝细胞癌（HCC）切除术后预后不良相关。然而，CD68染色不能区分原肿瘤或杀肿瘤亚群与泛巨噬细胞。CD163是替代性激活的巨噬细胞的标志物。在这项研究中，CD163+细胞在肿瘤和瘤周肝组织的临床意义进行了评估，在一个队列的295例肝癌根治性切除术后。我们发现，在肿瘤和癌旁肝组织中，CD 163+细胞密度与CD 68+细胞密度均呈良好的相关性，但两者的相关性更大。连续切片的免疫染色和手术切除标本的流式细胞术分析进一步支持了CD163+细胞比CD68+细胞更丰富的发现。肿瘤周围CD68+细胞密度与低无复发生存率（RFS）和低总生存率（OS）相关（分别为P= 0.004和P =0.001），而CD163+细胞在肿瘤或肿瘤周围肝组织中均无预后价值。 在另一组107例HCC患者中，术前血浆可溶性CD 163（sCD 163）浓度与活动性肝炎相关因子相关，但与肿瘤浸润标志物无关。总之，CD163+细胞局部浸润和血浆sCD163在HCC中的意义有限，它们更可能是与活动性肝炎相关的标志物，而不是肿瘤进展。
Our previous study has found that the abundance of peritumoral CD68+ macrophages was associated with poor prognosis in hepatocellular carcinoma (HCC) after resection. However, CD68 staining could not discriminate the protumoral or tumoricidal subpopulations from pan-macrophages. CD163 is a marker of alternatively activated macrophages. In this study, the clinical significance of CD163+ cells in tumors and peritumoral liver tissues was evaluated in a cohort of 295 patients with HCC after curative resection. We found that the density of CD163+ cells was well correlated with that of CD68+ cells in both tumors and peritumoral liver tissues but was much more. Immunostaining on consecutive sections and flow cytometry assay on surgical resected specimens further supported the findings that the CD163+ cells was more abundant than CD68+ cells. The density of peritumoral CD68+ cells was associated with poor recurrence-free survival (RFS) and poor overall survival (OS) (P = 0.004 and P = 0.001, respectively), whereas the CD163+ cells have no prognostic values either in tumors or in peritumoral liver tissues. In another cohort of 107 HCC patients, preoperative plasma concentration of soluble form of CD163 (sCD163) was associated with active hepatitis-related factors but not associated with the markers of tumor invasion. In conclusion, both the CD163+ cells local infiltration and plasma sCD163 were of limited significance in HCC, and they were more likely markers related to active hepatitis rather than tumor progression.
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