Persistence of fecal indicator bacteria in sediment of an oligotrophic river: comparing large and lab-scale flume systems.
Persistence of fecal indicator bacteria in sediment of an oligotrophic river: comparing large and lab-scale flume systems.
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贫营养河流沉积物中粪便指示菌的持久性：比较大型和实验室规模的水槽系统
DOI：
10.1016/j.watres.2014.05.007
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发表时间：
2014
期刊：
影响因子：
12.8
通讯作者：
Horn H.
中科院分区：
文献类型：
--
作者：
Walters E;Kätzl K;Müller E;Schwarzwälder K;Rutschmann P;Horn H.
在这项研究中，无论是实验室和大型水槽系统被用来调查粪便指示细菌（FIB）的生存在高山贫营养河流的河床沉积物。为了确定基质对持久性的影响，测试了3 cm深的基质笼内与薄的生物膜覆盖的瓷砖上的存活率。此外，还研究了床层剪切力对床层沉积物中存活率的影响，发现在床层剪切力非常低（0.3 N m−2）的实验室水槽中，大肠杆菌和肠球菌在3 cm深的底层笼中的存活率与在薄生物膜（200 μm）中的几乎相同。然而，在床剪切应力为9 N m−2的大型水槽系统中，较深底层笼的额外保护比薄生物膜促进大肠杆菌和肠球菌的存活时间长得多。此外，FIB去除机制在两个水槽系统不同。在实验室规模中，肠球菌的存活时间是大肠杆菌的两倍，而在大规模水槽中，两种FIB的去除率相同。在两个水槽进行的qPCR分析的比较表明，床沉积物侵蚀和食草动物/捕食者的影响负责FIB从沉积物中的大型水槽去除，而在实验室水槽FIB失活造成去除。这些结果表明，水力参数，如床面剪切应力以及系统中的大型无脊椎动物的存在下，设计水槽时要考虑的两个重要因素，因为它们可以显着影响FIB的持久性在沉积物中的快速流动，高山溪流。
In this study, both a lab and a large-scale flume system were used to investigate the survival of fecal indicator bacteria (FIB) in bed sediments of an alpine oligotrophic river. To determine the influence of substratum on persistence, survival within 3-cm-deep substratum cages versus on thin, biofilm-covered ceramic tiles was tested. Moreover, the impact of bed shear stress on survival in bed sediments was explored.It was seen that in the lab-scale flume having a very low bed shear stress (0.3 N m−2),E.coliand enterococci survival in 3-cm-deep substratum cages was nearly the same as in a thin biofilm (200 μm). However, in the large-scale flume system characterized by a bed shear stress of 9 N m−2, the added protection of the deeper substratum cages promoted considerably longer survival ofE.coliand enterococci than the thin biofilm. Additionally, the FIB removal mechanisms in the two flume systems varied. At the lab-scale, enterococci was seen to persist twice as long asE.coli, while in the large-scale flume the two FIB were removed at the same rate. A comparison of qPCR analyses performed in both flumes suggests that bed sediment erosion and the influence of grazers/predators were responsible for FIB removal from the sediments in the large-scale flume, whereas in the lab flume FIB inactivation caused removal. These results indicate that hydraulic parameters such as bed shear stress as well as the presence of macroinvertebrates in a system are both important factors to consider when designing flumes as they can significantly impact FIB persistence in sediments of fast-flowing, alpine streams.
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影响因子：
12.8
作者：
Lee, Christine M.;Lin, Tiffany Y.;Jay, Jennifer A.
通讯作者：
Jay, Jennifer A.
影响因子：
5.1
作者：
Reischer GH;Haider JM;Sommer R;Stadler H;Keiblinger KM;Hornek R;Zerobin W;Mach RL;Farnleitner AH
通讯作者：
Farnleitner AH
影响因子：
4.4
作者：
C. Gerba;John S. McLEOD
通讯作者：
John S. McLEOD
重悬浮对模拟寡营养河流的大型水槽中粪便指示菌命运的影响
影响因子：
12.8
作者：
Walters E;Müller E;Schwarzwälder K;Kätzl K;Rutschmann P;Horn H.
通讯作者：
Horn H.
粪便细菌
DOI：
10.1128/9781555816865
发表时间：
2011
期刊：
Applied microbiology
影响因子：
--
作者：
M. Sadowsky;R. Whitman
通讯作者：
R. Whitman