Biochemical and Comparative Transcriptomic Analyses Identify Candidate Genes Related to Variegation Formation in Paeonia rockii.
Biochemical and Comparative Transcriptomic Analyses Identify Candidate Genes Related to Variegation Formation in Paeonia rockii.
复制标题
DOI：
10.3390/molecules22081364
复制
发表时间：
2017-08-17
期刊：
影响因子：
--
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Shi Q;Li L;Zhang X;Luo J;Li X;Zhai L;He L;Zhang Y
牡丹是一种野生牡丹，花瓣底部有大而深的紫色斑纹。它是牡丹品种各种花纹的遗传资源，与牡丹纯白的花瓣形成鲜明对比。然而，这种植物杂色形成的分子机制仍不清楚。在这里，我们对罗氏、奥斯提埃(花瓣为纯白色)及其F1个体(花瓣为紫红色)进行了Illumina转录组测序。共产生181,866个Uniges，其中包括参与花青素生物合成和封存以及花青素生物合成调控的各种Uniges。暗紫色或紫红色斑纹的出现主要是由于花青素(Cy)和牡丹素(Pn)花色苷的比例所致。岩松杂交种表现为“Cy>；Pn”表型，而F1代表现为“Pn>；Cy”表型。根据基因表达和相互作用网络分析，CHS、DFR、ANS和GST基因可能在罗氏松杂色着色中起关键作用。两个R2R3-MYB转录因子(c131300.graph_c0和c133735.graph_c0)通过控制CHS、ANS和GST基因来调控变异的形成。我们的结果表明，不同的花色模式是由花青素生物合成基因的转录调控引起的，R2R3-MYB的转录图谱为阐明这一特性的机制提供了线索。本研究获得的花瓣转录组数据将为今后牡丹不同变异模式遗传调控的关联研究提供有价值的资源。
Paeonia rockii is a wild tree peony species with large and dark purple variegations at the base of its petals. It is the genetic resource for various variegation patterns in tree peony cultivars, which is in contrast to the pure white petals of Paeonia ostii. However, the molecular mechanism underlying the formation of variegation in this plant is still unknown. Here, we conducted Illumina transcriptome sequencing for P. rockii, P. ostii (with pure white petals) and their F1 individuals (with purple-red variegation). A total of 181,866 unigenes were generated, including a variety of unigenes involved in anthocyanin biosynthesis and sequestration and the regulation of anthocyanin biosynthesis. The dark purple or purple-red variegation patterns mainly occurred due to the proportions of cyanidin (Cy)- and peonidin (Pn)-based anthocyanins. The variegations of P. rockii exhibited a “Cy > Pn” phenotype, whereas the F1 progeny showed a “Pn > Cy” phenotype. The CHS, DFR, ANS, and GST genes might play key roles in variegation pigmentation in P. rockii according to gene expression and interaction network analysis. Two R2R3-MYB transcription factors (c131300.graph_c0 and c133735.graph_c0) regulated variegation formation by controlling CHS, ANS and GST genes. Our results indicated that the various variegation patterns were caused by transcriptional regulation of anthocyanin biosynthesis genes, and the transcription profiles of the R2R3-MYBs provided clues to elucidate the mechanisms underlying this trait. The petal transcriptome data produced in this study will provide a valuable resource for future association investigations of the genetic regulation of various variegation patterns in tree peonies.
登录
查看更多内容
影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
影响因子：
3.7
作者：
Shi Q;Zhou L;Wang Y;Li K;Zheng B;Miao K
通讯作者：
Miao K
影响因子：
4.9
作者：
Shoji, Kazuaki;Miki, Naoko;Yoshida, Kumi
通讯作者：
Yoshida, Kumi
影响因子：
64.8
作者：
NODA, K;GLOVER, BJ;MARTIN, C
通讯作者：
MARTIN, C