Birthdate study of GABAergic neurons in the lumbar spinal cord of the glutamic acid decarboxylase 67-green fluorescent protein knock-in mouse.
Birthdate study of GABAergic neurons in the lumbar spinal cord of the glutamic acid decarboxylase 67-green fluorescent protein knock-in mouse.
复制标题
谷氨酸脱羧酶67-绿色荧光蛋白敲入小鼠腰脊髓GABA能神经元的出生日期研究
DOI：
10.3389/fnana.2013.00042
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发表时间：
2013
影响因子：
2.9
通讯作者：
Wu SX
中科院分区：
文献类型：
--
作者：
Huang J;Chen J;Wang W;Wei YY;Cai GH;Tamamaki N;Li YQ;Wu SX
尽管对γ-氨基丁酸（GABA）能神经元在小鼠脊髓发育中的分布有大量的研究，但迄今为止还没有对其出生日期进行研究。为了确定特定表型的脊髓神经发生，脊髓中的GABA能神经元，我们在谷氨酸脱羧酶（GAD）67-绿色荧光蛋白（GFP）基因敲入小鼠的不同发育阶段注射溴脱氧尿苷（BrdU）。因此，我们使用GFP标记GABA能神经元，并用S期标记物BrdU标记不同胚胎阶段的新生细胞。然后在60日龄小鼠脊髓切片中研究BrdU标记的GABA能神经元的分布。我们的出生日期研究表明，GABA能神经发生在胚胎10.5天（E10.5）。此后，GABA能神经元的产生显著增加，并在E11.5达到高峰。在本研究中，在E11.5和E13.5处观察到GABA和BrdU在脊髓中的共定位的两个波。绝大多数GABA能神经元在E14.5之前产生。此后，GABA阳性神经元的产生急剧减少。结果表明，各板层GABA能神经元的出生日期不同。GABA能神经发生的高峰在Ⅱ层的E11.5和E13.5，而在Ⅰ层和Ⅲ层，它们分别在E13.5和E12.5。结果提示，GABA能神经元的出生时间在不同的板层中不同，并遵循特定的时间顺序。这将为今后的功能研究提供有价值的信息。
Despite the abundance of studies on γ-aminobutyric acid (GABA) ergic neuron distribution in the mouse developing spinal cord, no investigation has been devoted so far to their birthdates. In order to determine the spinal neurogenesis of a specific phenotype, the GABAergic neurons in the spinal cord, we injected bromodeoxyuridine (BrdU) at different developmental stages of the glutamic acid decarboxylase (GAD)67-green fluorescent protein (GFP) knock-in mice. We thus used GFP to mark GABAergic neurons and labeled newly born cells with the S-phase marker BrdU at different embryonic stages. Distribution of GABAergic neurons labeled with BrdU was then studied in spinal cord sections of 60-day-old mice. Our birthdating studies revealed that GABAergic neurogenesis was present at embryonic day 10.5 (E10.5). Since then, the generation of GABAergic neurons significantly increased, and reached a peak at E11.5. Two waves for the co-localization of GABA and BrdU in the spinal cord were seen at E11.5 and E13.5 in the present study. The vast majority of GABAergic neurons were generated before E14.5. Thereafter, GABA-positive neuron generation decreased drastically. The present results showed that the birthdates of GABAergic neurons in each lamina were different. The peaks of GABAergic neurogenesis in lamina II were at E11.5 and E13.5, while in lamina I and III, they were at E13.5 and E12.5, respectively. The present results suggest that the birthdates of GABAergic neurons vary in different lamina and follow a specific temporal sequence. This will provide valuable information for future functional studies.
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影响因子：
5.3
作者：
Branchereau, P;Chapron, J;Meyrand, P
通讯作者：
Meyrand, P
影响因子：
2.5
作者：
MCLAUGHLIN, BJ;BARBER, R;WU, JY
通讯作者：
WU, JY
影响因子：
2.9
作者：
MILLER, MW;NOWAKOWSKI, RS
通讯作者：
NOWAKOWSKI, RS
影响因子：
5.5
作者：
Rivera, C;Voipio, J;Kaila, K
通讯作者：
Kaila, K
影响因子：
--
作者：
ABERCROMBIE, M
通讯作者：
ABERCROMBIE, M