Screening for opioids in dog heart.
Screening for opioids in dog heart.
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筛查狗心脏中的阿片类药物。
DOI：
10.1016/0022-2828(92)91160-7
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发表时间：
1992
影响因子：
5
通讯作者：
Caffrey,JL
中科院分区：
文献类型：
--
作者：
Barron,BA;Gu,H;Gaugl,JF;Caffrey,JL
将狗心脏分为右心房和左心房、右心室和左心室以及心室间隔在酸中均质化以便提取。分别通过放射受体和放射免疫测定法测定总阿片类药物和特定肽（甲硫氨酸-脑啡肽、甲硫氨酸-脑啡肽-arg6-gly7-leu8）。儿茶酚胺按照 HPLC 进行安培定量。评估了麻醉剂（戊巴比妥、α-氯醛糖）、出血和神经节阻滞（六甲铵和阿托品）的影响。总阿片类药物、脑啡肽和肾上腺素均匀分布在整个心肌中，而去甲肾上腺素优先集中在心房中。通过放射受体测定估计，免疫反应性蛋氨酸-脑啡肽仅占强心阿片类药物总量的 1% 至 2%。出血降低了整个心肌的蛋氨酸脑啡肽含量，但对总阿片类药物或儿茶酚胺没有显着影响。神经节阻断增加了总阿片类药物、蛋氨酸-脑啡肽-arg6-gly7-leu8和儿茶酚胺含量，但不改变蛋氨酸-脑啡肽含量。左心室提取物的 HPLC 表明，50% 的甲硫氨酸脑啡肽免疫反应性在与合成甲硫氨酸脑啡肽相同的保留时间内洗脱。总之，犬心肌内似乎存在大量阿片类药物浓度，其对生理和药物干预有反应。这些心脏阿片类药物反应与相同条件下儿茶酚胺观察到的变化并不平行。
Dog hearts divided into right and left atria, right and left ventricles and intraventricular septum were homogenized in acid for extraction. Total opioids, and specific peptides (methionine-enkephalin, methionine-enkephalin-arg6-gly7-leu8) were determined by radioreceptor and radioimmunoassay, respectively. Catecholamines were quantitated amperometrically following HPLC. The effects of anesthetic agents (pentobarbital, α-chloralose), hemorrhage and ganglionic blockade (hexamethonium and atropine) were evaluated. Total opioids, enkephalins and epinephrine were distributed uniformly throughout the myocardium, while norepinephrine was preferentially concentrated in the atria. Immunoreative methionine-enkephalin accounted for only 1 to 2% of the total cardiac opioids estimated by radioreceptor assay. Hemorrhage lowered methionine-enkephalin content throughout the myocardium with no significant effect on total opioids or catecholamines. Ganglionic blockade increased total opioid, methionine-enkephalin-arg6-gly7-leu8and catecholamine content without altering methionine-enkephalin content. HPLC of left ventricular extracts demonstrated that 50% of met-enkephalin-immunoreactivity eluted at retention times equal to synthetic met-enkephalin. In summary, there appears to be substantive opioid concentrations within canine myocardium which respond to physiological and pharmacological interventions. These cardiac opioid responses do not parallel changes observed for catecholamines under the same conditions.
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Met-脑啡肽-Arg6-Phe7在大鼠和豚鼠各组织中的分布
影响因子：
4.7
作者：
J. Tang;H. Y. Yang;E. Costa
通讯作者：
E. Costa
DOI：
--
发表时间：
1983
期刊：
Life Science
影响因子：
--
作者：
R. Lang;K. Hermann;R. Dietz;W. Gaida;D. Ganten;K. Kraft;T. Unger
通讯作者：
T. Unger
影响因子：
7.3
作者：
J. Hughes;H. Kosterlitz;T. Smith
通讯作者：
J. Hughes;H. Kosterlitz;T. Smith
影响因子：
2.2
作者：
ALLGOOD, SC;GURLL, NJ;REYNOLDS, DG
通讯作者：
REYNOLDS, DG
出血和纳洛酮对氟烷麻醉犬肾上腺释放甲硫氨酸脑啡肽和儿茶酚胺的影响。
DOI：
10.1016/0165-1838(87)90089-0
发表时间：
1987
期刊：
Journal of the autonomic nervous system
影响因子：
--
作者：
Gaumann,DM;Yaksh,TL;Dousa,MK;Tyce,GM;Lucas,DL;Hench,VS
通讯作者：
Hench,VS