Isolation and Characterization of AGAMOUS-Like Genes Associated With Double-Flower Morphogenesis in Kerria japonica (Rosaceae).
Isolation and Characterization of AGAMOUS-Like Genes Associated With Double-Flower Morphogenesis in Kerria japonica (Rosaceae).
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与棣棠（蔷薇科）双花形态发生相关的 AGAMOUS 样基因的分离和表征
DOI：
10.3389/fpls.2018.00959
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发表时间：
2018
影响因子：
5.6
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Ma J;Shen X;Liu Z;Zhang D;Liu W;Liang H;Wang Y;He Z;Chen F
双花型植物在园林和观赏植物中更受欢迎。研究重瓣花形成的分子机制，为进一步的育种和选择提供理论依据。Kerria japonica是蔷薇科（Rosaceae）的一种观赏灌木，具有单花和双花两种类型，雄蕊可同源异型转化为花瓣，心皮可同源异型转化为叶状心皮，因此被认为是研究植物形态变化机制的理想系统。本研究以单花K. japonica）和Df-KjAG（重瓣K. japonica）分离到两个AGAMOUS（AG）同源物，它们严格存在于单花和双花K. japonica，分别。我们的序列比较表明，Df-KjAG是来自异位剪接插入2411 bp的转座子样片段，这可能会破坏mRNA的积累和蛋白质的功能，到内含子1。拟南芥中的异位表达分析表明，Sf-KjAG在指定心皮和雄蕊身份方面高度保守。然而，Df-KjAG没有显示任何推定的C类功能在花发育。此外，酵母双杂交试验表明，Sf-KjAG可以与KjAGL 2、KjAGL 9和KjAP 1相互作用，而Df-KjAG则失去了与这些花识别基因的相互作用。此外，Df-KjAG的功能丧失不仅影响其自身的表达，而且影响其他假定的花器官身份基因，如KjAGL 2，KjAGL 9，KjAP 1，KjAP 2，KjAP 3和KjPI的表达。因此，我们的研究结果表明，重瓣花的形成K。Df-KjAG是一个转座子样片段插入到单花K的正常AG同源物（Sf-KjAG）中而产生的突变体。日本。亮点：Sf-KjAG和Df-KjAG是两种不同的变异，它们之间的区别仅在于插入了一个转座子样片段，导致了Kerria japonica从单花形态向双花形态的进化转变。
Double-flower phenotype is more popular and attractive in garden and ornamental plants. There is great interest in exploring the molecular mechanisms underlying the double-flower formation for further breeding and selection. Kerria japonica, a commercial ornamental shrub of the Rosaceae family, is considered an excellent system to determine the mechanisms of morphological alterations, because it naturally has a single-flower form and double-flower variant with homeotic conversion of stamens into petals and carpels into leaf-like carpels. In this study, Sf-KjAG (AGAMOUS homolog of single-flower K. japonica) and Df-KjAG (AGAMOUS homolog of double-flower K. japonica) were isolated and characterized as two AGAMOUS (AG) homologs that occur strictly in single- and double-flower K. japonica, respectively. Our sequence comparison showed that Df-KjAG is derived from ectopic splicing with the insertion of a 2411 bp transposon-like fragment, which might disrupt mRNA accumulation and protein function, into intron 1. Ectopic expression analysis in Arabidopsis revealed that Sf-KjAG is highly conserved in specifying carpel and stamen identities. However, Df-KjAG did not show any putative C-class function in floral development. Moreover, yeast-two-hybrid assays showed that Sf-KjAG can interact with KjAGL2, KjAGL9, and KjAP1, whereas Df-KjAG has lost interactions with these floral identity genes. In addition, loss-of-function of Df-KjAG affected not only its own expression, but also that of other putative floral organ identity genes such as KjAGL2, KjAGL9, KjAP1, KjAP2, KjAP3, and KjPI. In conclusion, our findings suggest that double-flower formation in K. japonica can be attributed to Df-KjAG, which appears to be a mutant produced by the insertion of a transposon-like fragment in the normal AG homolog (Sf-KjAG) of single-flower K. japonica. Highlights: Sf-KjAG and Df-KjAG are different variations only distinguished by a transposon-like fragment insertion which lead to the evolutionary transformation from single-flower to double-flowers morphogenesis in Kerria japonica.
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DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
影响因子：
11.4
作者：
Davies, B;EgeaCortines, M;Sommer, H
通讯作者：
Sommer, H
影响因子：
7.4
作者：
Dong, Yiyu;Burch-Smith, Tessa M.;Dinesh-Kumar, Savithramma P.
通讯作者：
Dinesh-Kumar, Savithramma P.
影响因子：
7
作者：
El Baidouri M;Carpentier MC;Cooke R;Gao D;Lasserre E;Llauro C;Mirouze M;Picault N;Jackson SA;Panaud O
通讯作者：
Panaud O
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF