Composition based strategies for controlling radii in lipid nanotubes.
Composition based strategies for controlling radii in lipid nanotubes.
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DOI：
10.1371/journal.pone.0081293
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Cans AS
中科院分区：
文献类型：
--
作者：
Kurczy ME;Mellander LJ;Najafinobar N;Cans AS
大自然通常在脂质结合的细胞和细胞器内进行小规模化学反应。许多研究人员正在开发脂质体-脂质纳米管网络，试图模仿这些膜封闭的环境，目标是进行小规模的化学研究。这些系统的特征在于它们所构成的巨型单层囊泡的直径和连接它们的纳米管的长度。在这里，我们评估了两种基于固有曲率来调整纳米管直径的方法，这是网络先前无法控制的一个方面。这是通过两种不同的方法改变网络膜的脂质组成来完成的。第一种方法是通过外源脂质的脂质孵育来改变膜的组成；添加低固有曲率脂质大豆磷脂酰胆碱 (soy-PC) 或高固有曲率脂质大豆磷脂酰乙醇胺 (soy-PE)。在第二种方法中，在脂质体形成过程中将外源脂质添加到总脂质组合物中。在这里，我们表明，对于两种脂质增强方法，我们观察到添加大豆-PE 后纳米管直径减小，但添加大豆-PC 后尺寸没有显着变化。我们的结果表明，大豆-PE 对纳米管直径的影响与添加方法无关，并且表明高曲率大豆-PE 分子有利于管膜弯曲。
Nature routinely carries out small-scale chemistry within lipid bound cells and organelles. Liposome–lipid nanotube networks are being developed by many researchers in attempt to imitate these membrane enclosed environments, with the goal to perform small-scale chemical studies. These systems are well characterized in terms of the diameter of the giant unilamellar vesicles they are constructed from and the length of the nanotubes connecting them. Here we evaluate two methods based on intrinsic curvature for adjusting the diameter of the nanotube, an aspect of the network that has not previously been controllable. This was done by altering the lipid composition of the network membrane with two different approaches. In the first, the composition of the membrane was altered via lipid incubation of exogenous lipids; either with the addition of the low intrinsic curvature lipid soy phosphatidylcholine (soy-PC) or the high intrinsic curvature lipid soy phosphatidylethanolamine (soy-PE). In the second approach, exogenous lipids were added to the total lipid composition during liposome formation. Here we show that for both lipid augmentation methods, we observed a decrease in nanotube diameter following soy-PE additions but no significant change in size following the addition of soy-PC. Our results demonstrate that the effect of soy-PE on nanotube diameter is independent of the method of addition and suggests that high curvature soy-PE molecules facilitate tube membrane curvature.
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DOI：
10.1073/pnas.0907354106
发表时间：
2009-12-29
影响因子：
11.1
作者：
Kamal, Marzuk M.;Mills, Deryck;Howard, Jonathon
通讯作者：
Howard, Jonathon
影响因子：
3.3
作者：
Karlsson, A;Sott, K;Orwar, O
通讯作者：
Orwar, O
DOI：
10.1073/pnas.232702599
发表时间：
2003-01-21
影响因子：
11.1
作者：
Cans, AS;Wittenberg, N;Ewing, A
通讯作者：
Ewing, A
DOI：
10.1051/jphys:01986004703050700
发表时间：
1986-03-01
期刊：
JOURNAL DE PHYSIQUE
影响因子：
--
作者：
LEIBLER, S
通讯作者：
LEIBLER, S
DOI：
10.1073/pnas.95.19.11199
发表时间：
1998-09-15
影响因子：
11.1
作者：
Spang, A;Matsuoka, K;Orci, L
通讯作者：
Orci, L