Body mass index affects proliferation and osteogenic differentiation of human subcutaneous adipose tissue-derived stem cells.
Body mass index affects proliferation and osteogenic differentiation of human subcutaneous adipose tissue-derived stem cells.
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DOI：
10.1186/1471-2121-14-34
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发表时间：
2013-08-07
期刊：
影响因子：
--
通讯作者：
Rowan BG
中科院分区：
文献类型：
--
作者：
Frazier TP;Gimble JM;Devay JW;Tucker HA;Chiu ES;Rowan BG
肥胖与患癌症和代谢综合征并发症的风险较高有关。脂肪组织是公认的内分泌器官，因为它影响许多生理功能，并含有脂肪组织来源的干细胞（ASCs）。ASCs可以分化为多种细胞系，因此可用于组织工程和再生医学。然而，供者的身体质量指数（BMI）是否会影响ASC功能的问题仍然存在。从不同bmi （BMI-ASCs）的患者中分离ASCs，范围在18.5-32.8之间。假设超重的BMI-ASCs在早期成脂和成骨潜能以及体外形成菌落的能力方面会受到更大的损害。通过CyQUANT增殖试验（基于细胞DNA含量的荧光测量）和集落形成试验评估，BMI与ASC增殖和集落形成电位呈负相关。通过Oil-Red-O染色，BMI与早期时间点（第7天）呈正相关，而与晚期时间点（第15天）呈正相关。茜素红染色和碱性磷酸酶RT-PCR结果表明，BMI升高导致细胞外基质ASC矿化受损，碱性磷酸酶mRNA表达降低。这些数据表明，BMI升高导致ASC增殖减少，并可能损害体外成骨能力；因此，BMI是选择用于临床应用的ASC供体时考虑的重要标准。
Obesity is associated with a higher risk of developing cancer and co-morbidities that are part of the metabolic syndrome. Adipose tissue is recognized as an endocrine organ, as it affects a number of physiological functions, and contains adipose tissue-derived stem cells (ASCs). ASCs can differentiate into cells of multiple lineages, and as such are applicable to tissue engineering and regenerative medicine. Yet the question of whether ASC functionality is affected by the donor’s body mass index (BMI) still exists. ASCs were isolated from patients having different BMIs (BMI-ASCs), within the ranges of 18.5-32.8. It was hypothesized that overweight BMI-ASCs would be more compromised in early adipogenic and osteogenic potential, and ability to form colonies in vitro. BMI was inversely correlated with ASC proliferation and colony forming potential as assessed by CyQUANT proliferation assay (fluorescence- based measurement of cellular DNA content), and colony forming assays. BMI was positively correlated with early time point (day 7) but not later time point (day 15) intracytoplasmic lipid accumulation as assessed by Oil-Red-O staining. Alizarin red staining and RT-PCR for alkaline phosphatase demonstrated that elevated BMI resulted in compromised ASC mineralization of extracellular matrix and decreased alkaline phosphatase mRNA expression. These data demonstrate that elevated BMI resulted in reduced ASC proliferation, and potentially compromised osteogenic capacity in vitro; thus BMI is an important criterion to consider in selecting ASC donors for clinical applications.
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通过糖皮质激素受体和AP-1串扰来调节人骨髓基质细胞增殖和分化能力。
影响因子：
6.2
作者：
Carcamo-Orive, Ivan;Gaztelumendi, Ainhoa;Delgado, Jesus;Tejados, Naiara;Dorronsoro, Akaitz;Fernandez-Rueda, Jon;Pennington, Daniel J.;Trigueros, Cesar
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Trigueros, Cesar
DOI：
10.2106/00004623-200408000-00002
发表时间：
2004-08-01
影响因子：
5.3
作者：
Foran, JRH;Mont, MA;Hungerford, DS
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影响因子：
15.9
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Klemm, Dwight J.
影响因子：
7.4
作者：
Dardeno, Tina A.;Chou, Sharon H.;Moon, Hyun-Seuk;Chamberland, John P.;Fiorenza, Christina G.;Mantzoros, Christos S.
通讯作者：
Mantzoros, Christos S.
影响因子：
4.5
作者：
de Girolamo, Laura;Lopa, Silvia;Brini, Anna T.
通讯作者：
Brini, Anna T.