An integrative multi-omics analysis based on disulfidptosis-related prognostic signature and distinct subtypes of clear cell renal cell carcinoma.
An integrative multi-omics analysis based on disulfidptosis-related prognostic signature and distinct subtypes of clear cell renal cell carcinoma.
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DOI：
10.3389/fonc.2023.1207068
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发表时间：
2023
影响因子：
4.7
通讯作者：
Qin, Chao
中科院分区：
文献类型：
--
作者：
Zhang, Dong;Zhang, Xi;Liu, Zhanpeng;Han, Tian;Zhao, Kai;Xu, Xinchi;Zhang, Xu;Ren, Xiaohan;Qin, Chao
透明细胞肾细胞癌（ccRCC）和双硫中毒之间的关系仍有待深入研究。我们进行了多个生物信息学分析，包括预后分析和聚类分析，使用R软件。此外，我们利用定量实时PCR来测量特定基因的RNA水平。通过CCK 8和集落形成测定评估ccRCC的增殖，同时使用transwell测定评估ccRCC细胞的侵袭和迁移。在这项研究中，利用来自多个ccRCC队列的数据，我们确定了导致双硫中毒的分子。我们对这些分子的预后和免疫作用进行了全面的研究。在二硫中毒相关代谢基因（DMG）中，LRPPRC、OXSM、GYS 1和SLC7A11与ccRCC患者预后显著相关。根据我们的特征，不同组的患者显示出不同水平的免疫浸润和不同的突变谱。此外，我们将患者分为两组，并确定了在ccRCC的发生和发展中起重要作用的多个功能通路。鉴于其在双硫中毒中的关键作用，我们对SLC7A11进行了进一步分析。我们的结果表明，具有高表达SLC7A11的ccRCC细胞表现出恶性表型。这些发现增强了我们对DMG在ccRCC中的潜在功能的理解。
The association between clear cell renal cell carcinoma (ccRCC) and disulfidoptosis remains to be thoroughly investigated. We conducted multiple bioinformatics analyses, including prognostic analysis and cluster analysis, using R software. Additionally, we utilized Quantitative Real-time PCR to measure RNA levels of specific genes. The proliferation of ccRCC was assessed through CCK8 and colony formation assays, while the invasion and migration of ccRCC cells were evaluated using the transwell assay. In this study, utilizing data from multiple ccRCC cohorts, we identified molecules that contribute to disulfidoptosis. We conducted a comprehensive investigation into the prognostic and immunological roles of these molecules. Among the disulfidoptosis-related metabolism genes (DMGs), LRPPRC, OXSM, GYS1, and SLC7A11 exhibited significant correlations with ccRCC patient prognosis. Based on our signature, patients in different groups displayed varying levels of immune infiltration and different mutation profiles. Furthermore, we classified patients into two clusters and identified multiple functional pathways that play important roles in the occurrence and development of ccRCC. Given its critical role in disulfidoptosis, we conducted further analysis on SLC7A11. Our results demonstrated that ccRCC cells with high expression of SLC7A11 exhibited a malignant phenotype. These findings enhanced our understanding of the underlying function of DMGs in ccRCC.
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