Interaction of yeast eIF4G with spliceosome components: implications in pre-mRNA processing events.
Interaction of yeast eIF4G with spliceosome components: implications in pre-mRNA processing events.
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DOI：
10.4161/rna.6.5.9861
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发表时间：
2009-11
期刊：
影响因子：
4.1
通讯作者：
Lewis J
中科院分区：
文献类型：
--
作者：
Kafasla P;Barrass JD;Thompson E;Fromont-Racine M;Jacquier A;Beggs JD;Lewis J
从哺乳动物细胞中证实，真核翻译起始因子eIF4G在核RNA代谢中具有假定的作用。在这里，我们研究这种作用是否在酵母酿酒酵母中保守。通过体外和体内结合的方法，我们发现，与哺乳动物eIF4G类似，酵母eIF4G的同源物Tif4631p和Tif4632p同时存在于细胞核和细胞质中。我们表明，这两种eIF4G蛋白在体外与剪接机制的UsnRNP组分有效地相互作用。更具体地说，Tif4631p和Tif4632p在体外有效地与U1 snRNA相互作用。此外，Tif4631p和Tif4632p与剪接机制的蛋白质组分，即Snu71p和Prp11p结合。为了进一步描述这些相互作用，我们绘制了Tif4631p和Tif4632p与Prp11p和Snu71p相互作用的重要区域，我们发现在体外剪接反应中添加这些区域具有主要的抑制作用。观察到的相互作用暗示eIF4G参与mrna前加工的各个方面。为了支持这一假设，eIF4G亚型之一的缺失会导致体内许多内源性基因的未剪接前体的积累。总之，这些观察结果提示酵母eIF4G参与mrna前代谢。
As evidenced from mammalian cells the eukaryotic translation initiation factor eIF4G has a putative role in nuclear RNA metabolism. Here we investigate whether this role is conserved in the yeast Saccharomyces cerevisiae. Using a combination of in vitro and in vivo methods, we show that, similar to mammalian eIF4G, yeast eIF4G homologues, Tif4631p and Tif4632p, are present both in the nucleus and the cytoplasm. We show that both eIF4G proteins interact efficiently in vitro with UsnRNP components of the splicing machinery. More specifically, Tif4631p and Tif4632p interact efficiently with U1 snRNA in vitro. In addition, Tif4631p and Tif4632p associate with protein components of the splicing machinery, namely Snu71p and Prp11p. To further delineate these interactions, we map the regions of Tif4631p and Tif4632p that are important for the interaction with Prp11p and Snu71p and we show that addition of these regions to splicing reactions in vitro has a dominant inhibitory effect. The observed interactions implicate eIF4G in aspects of pre-mRNA processing. In support of this hypothesis, deletion of one of the eIF4G isoforms results in accumulation of un-spliced precursors for a number of endogenous genes, in vivo. In conclusion these observations are suggestive of the involvement of yeast eIF4G in pre-mRNA metabolism.
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影响因子：
11.4
作者：
Barrass, JD;Beggs, JD
通讯作者：
Beggs, JD
DOI：
10.1073/pnas.0400933101
发表时间：
2004-03-23
影响因子：
11.1
作者：
Ferraiuolo, MA;Lee, CS;Sonenberg, N
通讯作者：
Sonenberg, N
影响因子：
4.5
作者：
Baron-Benhamou, J;Fortes, P;Hentze, MW
通讯作者：
Hentze, MW
影响因子：
16
作者：
Fortes, P;Inada, T;Sachs, AB
通讯作者：
Sachs, AB
影响因子：
5.3
作者：
GOYER, C;ALTMANN, M;SONENBERG, N
通讯作者：
SONENBERG, N