Epitopes of glycoprotein G of equine herpesviruses 4 and 1 located near the C termini elicit type-specific antibody responses in the natural host
Epitopes of glycoprotein G of equine herpesviruses 4 and 1 located near the C termini elicit type-specific antibody responses in the natural host
复制标题
马疱疹病毒 4 和 1 的糖蛋白 G 位于 C 末端附近的表位在自然宿主中引发类型特异性抗体反应
作者：
B. Crabb;M. Studdert
马疱疹病毒4型（EHV 4;马疱疹病毒）和EHV 1（马流产病毒）的特异性血清学诊断迄今为止还不可能，因为这两种密切相关但不同的病毒之间存在广泛的抗原交叉反应性。最近，我们鉴定了EHV 4糖蛋白G（gG），并将其表征为型特异性分泌糖蛋白（B）。S.克拉布，H. S. Nagesha和M. Studdert，Virology 190：143-154，1992）。本文表明EHV 1 gG也具有型特异性表位，并描述了强型特异性表位定位于包括EHV 4 gG的氨基酸287至382和EHV 1 gG的氨基酸288至350的明显对应的高度可变区。表达这些可变区的融合蛋白与来自四匹马驹的血清反应强烈且类型特异，其中三匹是初乳剥夺的、无特异性病原体的马驹，其暴露于EHV 4或EHV 1的历史是明确的。这些抗原为开发单孔诊断性酶联免疫吸附试验以区分EHV 4、EHV 1或两者感染的马提供了基础。这种类型特异性试验首次提供了区分这些病毒抗体的机会，因此，它对了解这些马病原体的流行病学具有重要意义。证据表明，存在EHV 1在澳大利亚10年前的第一例确诊病例EHV 1流产。
Specific serological diagnosis of equine herpesvirus 4 (EHV4; equine rhinopneumonitis virus) and EHV1 (equine abortion virus) hitherto has not been possible because of extensive antigenic cross-reactivity between these two closely related but distinct viruses. Recently, we identified EHV4 glycoprotein G (gG) and characterized it as a type-specific, secreted glycoprotein (B. S. Crabb, H. S. Nagesha, and M. J. Studdert, Virology 190:143-154, 1992). This paper shows that EHV1 gG also possesses type-specific epitopes and describes the localization of strong, type-specific epitopes to the apparently corresponding and highly variable regions comprising amino acids 287 to 382 of EHV4 gG and 288 to 350 of EHV1 gG. Fusion proteins expressing these variable regions reacted strongly and type specifically with sera from four foals, three of which were colostrum-deprived, specific-pathogen-free foals, whose history with respect to exposure to EHV4 or EHV1 was well-defined. These antigens provided the basis for the development of a single-well diagnostic enzyme-linked immunosorbent assay to distinguish horses infected with EHV4, EHV1, or both. Such a type-specific test provides for the first time the opportunity to differentiate antibodies to these viruses, and it has, therefore, important implications for understanding the epidemiology of these equine pathogens. Evidence for the existence of EHV1 in Australia 10 years prior to the first confirmed case of EHV1 abortion is presented.
通过限制性核酸内切酶区分马疱疹病毒 1 的呼吸道和致流产分离株。
DOI：
10.1126/science.6270790
发表时间：
1981
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Studdert,MJ;Simpson,T;Roizman,B
通讯作者：
Roizman,B