Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice
Mosaic nanoparticles elicit cross-reactive immune responses to zoonotic coronaviruses in mice
复制标题
马赛克纳米颗粒在小鼠体内引发对人畜共患冠状病毒的交叉反应免疫反应
DOI：
10.1101/2020.11.17.387092
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Cohen A
中科院分区：
文献类型：
--
作者：
Cohen A
Protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and SARS-related emergent zoonotic coronaviruses is urgently needed. We made homotypic nanoparticles displaying the receptor binding domain (RBD) of SARS-CoV-2 or co-displaying SARS-CoV-2 RBD along with RBDs from animal betacoronaviruses that represent threats to humans (mosaic nanoparticles with four to eight distinct RBDs). Mice immunized with RBD nanoparticles, but not soluble antigen, elicited cross-reactive binding and neutralization responses. Mosaic RBD nanoparticles elicited antibodies with superior cross-reactive recognition of heterologous RBDs relative to sera from immunizations with homotypic SARS-CoV-2–RBD nanoparticles or COVID-19 convalescent human plasmas. Moreover, after priming, sera from mosaic RBD–immunized mice neutralized heterologous pseudotyped coronaviruses as well as or better than sera from homotypic SARS-CoV-2–RBD nanoparticle immunizations, demonstrating no loss of immunogenicity against particular RBDs resulting from co-display. A single immunization with mosaic RBD nanoparticles provides a potential strategy to simultaneously protect against SARS-CoV-2 and emerging zoonotic coronaviruses.
DOI：
10.1073/pnas.85.12.4526
发表时间：
1988-06-01
影响因子：
11.1
作者：
VLASAK, R;LUYTJES, W;PALESE, P
通讯作者：
PALESE, P
影响因子：
64.5
作者：
Hoffmann, Markus;Kleine-Weber, Hannah;Poehlmann, Stefan
通讯作者：
Poehlmann, Stefan