Cloning and characterization of an accessory gene regulator (agr)-like locus from Staphylococcus epidermidis.
Cloning and characterization of an accessory gene regulator (agr)-like locus from Staphylococcus epidermidis.
复制标题
表皮葡萄球菌辅助基因调节因子 (agr) 样基因座的克隆和表征。
作者：
W. V. Wamel;Gerda S.A.T van Rossum;J. Verhoef;C. Vandenbroucke;A. Fluit
通过琼脂糖基转移酶特异的聚合酶链式反应和Southern印迹杂交，证实了金黄色葡萄球菌的agr相关序列的存在。对表皮葡萄球菌A086的agr基因进行了克隆和序列分析。表皮葡萄球菌的agr基因座与金黄色葡萄球菌的agr基因座的同源性为68%。表皮葡萄球菌的agr基因座与金黄色葡萄球菌和鲁顿葡萄球菌的相同。推测的RNAII分子含有4个开放阅读框：agrA、B、C和D。agrA是一种反应调节因子。AgrB与转导分子和转位酶分子具有同源性。AGRC被认为是一种亮氨酸拉链存在的组氨酸蛋白激酶。据推测，AgD被加工成一种自诱导肽。推测的RNAIII分子含有一个开放阅读框，编码26个氨基酸(AA)的多肽，与编码金黄色葡萄球菌Delta毒素的RNAIII分子有3个氨基酸的差异。动力学研究表明，该RNAIII的产量在指数后阶段升高。23株表皮葡萄球菌中有21株具有Delta毒素活性。对Delta型毒素产生的动力学研究表明，该毒素是在指数后阶段产生的。对表皮葡萄球菌A097的测序显示了一个延迟的agr反应，显示了一个缺失组氨酸激酶域的截短的agrC。这些数据表明，在指数期后阶段，表皮葡萄球菌中存在一个类似agr的基因。
The presence of sequences related to the agr of Staphylococcus aureus was demonstrated in Staphylococcus epidermidis by agr-specific PCR, and Southern blot. The agr-like locus of S. epidermidis A086 was cloned and sequenced. An overall homology of 68% was found between the agr locus from S. epidermidis and S. aureus. The agr locus from S. epidermidis was organized similar to those from S. aureus and S. lugdunensis. The putative RNAII molecule contains four open reading frames, agr A, B, C and D. AgrA was a response regulator. AgrB showed homology with transducer and translocase molecules. AgrC is expected to act as a histidine protein kinase in which a leucine zipper is present. AgrD is presumably processed into an autoinducer peptide. The putative RNAIII molecule contained an open reading frame encoding a putative 26 amino acid (aa) polypeptide, which differed in 3 aa from the RNAIII encoded delta-toxin of S. aureus. Kinetic studies showed that the production of this RNAIII was elevated during the post-exponential phase. delta-Toxin activity was demonstrated for 21 of 23 tested S. epidermidis strains. Kinetic studies of the production of delta-toxin showed that the toxin was produced during the post-exponential phase. Sequencing of S. epidermidis A097, which showed a delayed agr-response, revealed a truncated AgrC lacking the histidine kinase domain. These data indicate that an agr-like locus is active in S. epidermidis during the post-exponential phase.
影响因子：
56.9
作者：
Ji, GY;Beavis, R;Novick, RP
通讯作者：
Novick, RP