A Microsatellite-Based Genetic Linkage Map of the Cichlid Fish, Astatotilapia burtoni (Teleostei): A Comparison of Genomic Architectures Among Rapidly Speciating Cichlids
A Microsatellite-Based Genetic Linkage Map of the Cichlid Fish, Astatotilapia burtoni (Teleostei): A Comparison of Genomic Architectures Among Rapidly Speciating Cichlids
复制标题
基于微卫星的丽鱼科鱼 Astatotilapia burtoni (Teleostei) 遗传连锁图：快速形成丽鱼科鱼的基因组结构比较
作者：
M. Sanetra;F. Henning;S. Fukamachi;A. Meyer
慈鲷科鱼类组成了数量惊人的物种，并在创纪录的时间内形成了物种群。为了便于对丽鱼进行有效的基因组扫描和比较，我们构建了一张中等密度的伯氏丽罗非鱼微卫星遗传连锁图谱。利用两个自交系进行作图杂交，通过互交获得F2代。该图谱显示了25个连锁群，分布在1249.3 cM的基因组（大小为950 Mb），平均标记间距为6.12 cM。7个Hox簇、ParaHox C1和Pdgfrβ的两个旁系同源物被定位到不同的连锁群，从而支持硬骨鱼特异性基因组重复的假设。助理burtoni连锁图进行了比较，其他两个可用的地图慈鲷使用共享的标记，显示东非慈鲷基因组之间的保守性和共线性。感兴趣的候选基因慈鲷物种利用SNP标记进行了映射。
Cichlid fishes compose an astonishingly large number of species and formed species flocks in record-breaking time. To facilitate efficient genome scans and comparisons of cichlid genomes, we constructed a medium-density genetic linkage map of microsatellite markers of Astatotilapia burtoni. The mapping cross was derived from two inbred laboratory lines to obtain F2 progeny by intercrossing. The map revealed 25 linkage groups spanning 1249.3 cM of the genome (size ∼950 Mb) with an average marker spacing of 6.12 cM. The seven Hox clusters, ParaHox C1, and two paralogs of Pdgfrβ were mapped to different linkage groups, thus supporting the hypothesis of a teleost-specific genome duplication. The A. burtoni linkage map was compared to the other two available maps for cichlids using shared markers that showed conservation and synteny among East African cichlid genomes. Interesting candidate genes for cichlid speciation were mapped using SNP markers.
影响因子：
4.6
作者：
D. Parichy;J. Rawls;S. J. Pratt;T. Whitfield;Stephen L. Johnson
通讯作者：
D. Parichy;J. Rawls;S. J. Pratt;T. Whitfield;Stephen L. Johnson
影响因子：
3.5
作者：
ZHAO, ZY;LEE, CC;PATEL, PI
通讯作者：
PATEL, PI