Single-Particle Interferometric Reflectance Imaging Characterization of Individual Extracellular Vesicles and Population Dynamics.
Single-Particle Interferometric Reflectance Imaging Characterization of Individual Extracellular Vesicles and Population Dynamics.
复制标题
DOI：
10.3791/62988
复制
发表时间：
2022-01-10
期刊：
影响因子：
--
通讯作者：
Christenson LK
中科院分区：
文献类型：
--
作者：
Deng F;Ratri A;Deighan C;Daaboul G;Geiger PC;Christenson LK
细胞外囊泡（EV）是由大多数细胞分泌的具有脂质双层的纳米大小的囊泡。EV携带多种不同的生物分子，包括蛋白质、脂质、DNA和RNA，并且被认为促进不同组织和器官中的细胞间通讯。最近，EV作为用于各种疾病的诊断和治疗剂的生物标志物已经引起了极大的关注。已经开发了许多用于EV表征的方法。然而，目前的EV分析方法都有不同的局限性。因此，开发高效和有效的EV分离和表征方法仍然是这个尖端研究领域成熟的关键步骤之一。在这里，我们提供了一个详细的协议，概述了单粒子干涉反射成像传感器（SP-IRIS），作为一种方法，能够检测和表征电动汽车从未纯化的生物来源和纯化的电动汽车通过其他方法。这种先进的技术可用于多水平和全面的测量，用于分析EV大小、EV计数、EV表型和生物标志物共定位。该协议提出了单粒子干涉反射成像，其设计用于细胞外囊泡（EV）大小、EV计数、EV表型和EV生物标志物共定位的多水平和全面测量。
Extracellular vesicles (EVs) are nanometer-sized vesicles with a lipid bilayer that are secreted by most cells. EVs carry a multitude of different biological molecules, including protein, lipid, DNA, and RNA, and are postulated to facilitate cell-to-cell communication in diverse tissues and organs. Recently, EVs have attracted significant attention as biomarkers for diagnostics and therapeutic agents for various diseases. Many methods have been developed for EV characterization. However, current methods for EV analysis all have different limitations. Thus, developing efficient and effective methods for EV isolation and characterization remains one of the crucial steps for this cutting-edge research field as it matures. Here, we provide a detailed protocol outlining a single-particle interferometric reflectance imaging sensor (SP-IRIS), as a method that is capable of detecting and characterizing EVs from unpurified biological sources and purified EVs by other methodologies. This advanced technique can be used for multi-level and comprehensive measurements for the analysis of EV size, EV count, EV phenotype, and biomarker colocalization. This protocol presents single-particle interferometric reflectance imaging that is designed for the multi-level and comprehensive measurements of extracellular vesicles (EV) size, EV count, EV phenotype, and EV biomarker colocalization.
登录
查看更多内容
影响因子：
2.5
作者：
Pohler KG;Green JA;Moley LA;Gunewardena S;Hung WT;Payton RR;Hong X;Christenson LK;Geary TW;Smith MF
通讯作者：
Smith MF
DOI：
10.1039/c5an00688k
发表时间：
2015-10-07
期刊：
The Analyst
影响因子：
--
作者：
Wu Y;Deng W;Klinke DJ 2nd
通讯作者：
Klinke DJ 2nd
影响因子：
6
作者：
Sun B;Tang N;Peluso MJ;Iyer NS;Torres L;Donatelli JL;Munter SE;Nixon CC;Rutishauser RL;Rodriguez-Barraquer I;Greenhouse B;Kelly JD;Martin JN;Deeks SG;Henrich TJ;Pulliam L
通讯作者：
Pulliam L
影响因子：
16
作者：
Bachurski, Daniel;Schuldner, Maximiliane;von Strandmann, Elke Pogge
通讯作者：
von Strandmann, Elke Pogge
影响因子：
6.1
作者：
Lee，Mijung;Im，Wooseok;Kim，Manho
通讯作者：
Kim，Manho