ID1 As a Prognostic Biomarker and Promising Drug Target Plays a Pivotal Role in Deterioration of Clear Cell Renal Cell Carcinoma.
ID1 As a Prognostic Biomarker and Promising Drug Target Plays a Pivotal Role in Deterioration of Clear Cell Renal Cell Carcinoma.
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ID1 作为一种预后生物标志物和有前景的药物靶点在透明细胞肾细胞癌的恶化中发挥着关键作用
DOI：
10.1155/2020/2064582
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发表时间：
2020
影响因子：
--
通讯作者：
Li Q
中科院分区：
文献类型：
--
作者：
Qiu X;Gu Y;Ni Y;Li Q
透明细胞肾细胞癌（ccRCC）是世界上最常见的癌症之一。我们的目标是确定有助于早期ccRCC进展的预后生物标志物并阐明其机制。在此，ccRCC样品的mRNA微阵列表达谱获自Gene Expression Omnibus（GEO）（GSE 68417）。利用R Studio软件获得62个差异表达基因（DEG），其中上调基因31个，下调基因31个。在大卫数据库中进行途径富集分析。通过STRING数据库获得蛋白质相互作用网络，并利用Cytoscape进行可视化。随后，在网络中，只有DNA结合抑制剂1（ID 1）在TCGA数据集中的低级别和高级别ccRCC患者之间具有显著性。在R Studio中分析相应的临床信息后，显示ID 1低表达与生存率差、肿瘤转移概率高和相对高的血清钙相关。随后，在GeneMANIA中对ID 1进行功能富集，发现调控DNA结合是ccRCC中ID 1的主要特征，这一点通过使用KM数据库和R Studio对ID 1相关基因的Kaplan-Meier曲线进行验证。肿瘤免疫评估资源（TIMER）进行的免疫浸润分析显示，CD 8 + T细胞和巨噬细胞是预后因素。此外，丙戊酸被分析为比较毒理学基因组学数据库（CTD）确定的ID 1的最确信的靶药物。综上所述，ID 1是早期ccRCC患者临床结果的生物标志物，具有预防ccRCC进展和转移恶化的潜在功能。
Clear cell renal cell carcinoma (ccRCC) is one of the most common cancers in the world. Our aim is to identify prognostic biomarkers that contribute to the progression of early stage ccRCC and clarify the mechanism. Here, the mRNA microarray expression profile of ccRCC samples was obtained from Gene Expression Omnibus (GEO) (GSE68417). 62 differentially expressed genes (DEGs) were gained by R Studio, including 31 upregulated genes and 31 downregulated genes. Pathway enrichment analysis was performed in DAVID database. Then, the protein-protein interaction network was obtained through STRING database and visualized by Cytoscape. Subsequently, among the network, only inhibitor of DNA Binding 1 (ID1) was significant between low-grade and high-grade ccRCC patients in TCGA data set. After analysis of the corresponding clinical information in R Studio, it is shown that low ID1 expression correlated with poor survival, high probability of tumor metastasis, and relatively high serum calcium. Later, functional enrichment of ID1 in GeneMANIA uncovered that regulating DNA binding is a main characteristic of ID1 in ccRCC, which was validated by Kaplan-Meier curve of ID1 associated genes using KM plotter database and R Studio. Immune infiltration analysis performed by Tumor Immune Estimation Resource (TIMER) revealed that CD8+ T cells and macrophages were prognostic factors. Furthermore, Valproic acid was analyzed to be the most convinced target drug of ID1 identified by Comparative Toxicogenomics Database (CTD). Taken together, ID1, a biomarker of clinical outcome in early stage ccRCC patients, has the potential function of preventing deterioration in ccRCC progression and metastasis.
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影响因子：
12.4
作者：
Ha H;Debnath B;Neamati N
通讯作者：
Neamati N
影响因子：
14.9
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Mattingly CJ
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3.7
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影响因子：
37.3
作者：
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Smyth GK