Biochemical Mediators of Meningeal Inflammatory Response to Group B Streptococcus in the Newborn Piglet Model
Biochemical Mediators of Meningeal Inflammatory Response to Group B Streptococcus in the Newborn Piglet Model
复制标题
新生仔猪模型中 B 族链球菌脑膜炎症反应的生化介质
作者：
E. Ling;F. Noya;G. Ricard;K. Beharry;E. L. Mills;J. Aranda
摘要：在新生仔猪模型中研究了脑膜对III型B链球菌（GBS）的热灭活突变型未包囊菌株的炎症反应。脑室内接种GBS（109菌落形成单位当量）或盐水（对照）。在基线和接下来的24小时内进行连续的脑脊液测量，以检测细胞化学变化和肿瘤坏死因子（TNF）和前列腺素的产生。在单独的实验中，我们定义的时间过程中的早期变化在第一个6小时和剂量反应关系的范围内接种106至109集落形成单位当量。脑室内接种热灭活的未包封的GBS诱导了显著的白细胞增多，并在6小时内增加了蛋白质。在这些变化之前，TNF（最大值在2小时）和白藜芦醇（最大值在2-4小时）增加了几百倍。TNF的早期和急剧上升表明其在启动炎症级联反应中的关键作用。在研究范围内，炎症反应的程度随着细菌剂量的增加而增加。为了研究封装GBS在脑膜炎症诱导中的作用，我们比较了对未封装的突变菌株与对封装的亲本菌株的反应。封装的菌株产生的炎症变化要小得多，而且只有在高剂量的细菌下才能产生。GBS细胞壁似乎是引发炎症的主要细菌产物。脑室内注射热灭活的未包封的GBS，暴露细胞壁，可作为研究新生儿脑膜炎的有效模型。
ABSTRACT: The meningeal inflammatory response to a heat-killed mutant unencapsulated strain of type III group B Streptococcus (GBS) was studied in a newborn piglet model. GBS (109 colony-forming unit equivalents) or saline (control) was inoculated intraventricularly. Serial cerebrospinal fluid measurements were done at baseline and over the course of the next 24 h for cytochemical changes and production of tumor necrosis factor (TNF) and prostaglandins. In separate experiments, we defined the time course of early changes during the first 6 h and dose response relationship over a range of inocula 106 to 109 colony-forming unit equivalents. The intraventricular inoculation of the heat-killed unencapsulated GBS induced marked leukocytosis and increased protein by 6 h. These changes were preceded by a several hundredfold increase in TNF (maximum at 2 h) and prostaglandins (maximum at 2–4 h). The early and sharp rise in TNF suggests its pivotal role in initiating the inflammatory cascade. The magnitude of the inflammatory response increased with increasing bacterial dose over the range studied. To study the effect of encapsulation of GBS in the induction of meningeal inflammation, we compared the response to the unencapsulated mutant strain with that to the encapsulated parent strain. The encapsulated strain produced much smaller inflammatory changes, and only with high doses of bacteria. The GBS cell wall appeared to be the primary bacterial product triggering inflammation. Intraventricular injection of the heat-killed unencapsulated GBS with exposed cell wall can serve as a valid model for studying neonatal meningitis.
吲哚美辛对家兔实验性脑膜炎病理生理学的影响。
DOI：
10.1093/infdis/163.3.647
发表时间：
1991
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Tureen,JH;Täuber,MG;Sande,MA
通讯作者：
Sande,MA
非甾体抗炎药治疗实验性肺炎球菌脑膜炎。
DOI：
10.1093/infdis/155.5.985
发表时间：
1987
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Tuomanen,E;Hengstler,B;Rich,R;Bray,MA;Zak,O;Tomasz,A
通讯作者：
Tomasz,A
细菌性脑膜炎：病理生理学和治疗的最新进展。
影响因子：
39.2
作者：
Tunkel,AR;Wispelwey,B;Scheld,WM
通讯作者：
Scheld,WM