Establishing a physiological environment for visualized in vitro brain slice recordings by increasing oxygen supply and modifying aCSF content.
Establishing a physiological environment for visualized in vitro brain slice recordings by increasing oxygen supply and modifying aCSF content.
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DOI：
10.1016/j.jneumeth.2009.06.005
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发表时间：
2009-10-15
影响因子：
3
通讯作者：
Mody, Istvan
中科院分区：
文献类型：
--
作者：
Hajos, Norbert;Mody, Istvan
我们的洞察神经元功能的基本特征显着先进的结合在体外切片技术与可视化的神经元及其过程。通过水浸物镜的可视化需要将切片保持浸没在记录室中，其中以2-3 ml/min的流速输送人工海马-脊髓液（aCSF）导致有限的氧气供应[Hájos N，Ellender TJ，Zemankovics R，Mann EO，Exley R，Cragg SJ等人，在浸没的海马切片中保持网络活动：氧气供应的重要性。Eur J Neurosci 2009;29：319-27]。在这里，我们回顾了两种方法，旨在提供足够的氧气水平的神经元淹没切片，使高耗能的过程，如提高发射率或网络振荡。这些方法的使用也可能影响其他电生理学实验的结果，包括淹没切片的细胞间信号通路的研究。此外，我们还强调了在体外实验中使用的各种aCSF成分的重要性。
Our insights into the basic characteristics of neuronal function were significantly advanced by combining the in vitro slice technique with the visualization of neurons and their processes. The visualization through water immersion objectives requires keeping slices submerged in recording chambers where delivering artificial cerebro-spinal fluid (aCSF) at flow rates of 2–3 ml/min results in a limited oxygen supply [Hájos N, Ellender TJ, Zemankovics R, Mann EO, Exley R, Cragg SJ, et al. Maintaining network activity in submerged hippocampal slices: importance of oxygen supply. Eur J Neurosci 2009;29:319–27]. Here we review two methods aimed at providing sufficient oxygen levels to neurons in submerged slices to enable high energy consuming processes such as elevated firing rates or network oscillations. The use of these methods may also influence the outcome of other electrophysiological experiments in submerged slices including the study of intercellular signaling pathways. In addition, we also emphasize the importance of various aCSF constituents used in in vitro experiments.
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影响因子：
2.5
作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.0600604103
发表时间：
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影响因子：
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作者：
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影响因子：
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作者：
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