Preparation of ethylene gas and comparison of ethylene responses induced by ethylene, ACC, and ethephon.
Preparation of ethylene gas and comparison of ethylene responses induced by ethylene, ACC, and ethephon.
复制标题
乙烯气体的制备以及乙烯、ACC 和乙烯利诱导的乙烯反应的比较。
DOI：
10.1016/j.plaphy.2009.10.002
复制
发表时间：
2010
影响因子：
6.5
通讯作者：
文啟光
中科院分区：
文献类型：
--
作者：
文啟光
乙烯是第一个被发现的调节植物生长发育许多方面的气体激素。在没有乙烯的情况下，ACC和乙烯利是两种广泛使用的替代乙烯处理的化学品。然而，由ACC和乙烯利转化的乙烯量是不可控制的，因此对于对乙烯浓度、响应窗口和处理时间敏感的实验来说，这两种处理方法是否能模拟乙烯的影响是值得怀疑的。乙烯可以通过乙醇脱水化学合成；然而，需要对脱水产物进行进一步的纯化。我们以前报道过在缓冲条件下分解乙烯可以很容易地制备乙烯气体，所得乙烯可以直接使用。乙烯响应可以通过测量黄化幼苗的下胚轴长度或通过erf1（乙烯响应因子1）的表达来估计。虽然低浓度的ACC不足以诱导erf1的表达，但高浓度的ACC可以在乙烯处理不可行的实验中替代乙烯。然而，ACC可能经历早期消耗。开发了多种方法，使缺乏乙烯和气体处理技术的实验室可以轻松地进行必要的乙烯处理。
Ethylene is the first identified gaseous hormone regulating many aspects of plant growth and development. ACC and ethephon are two widely used chemicals replacing ethylene treatment when ethylene is not available. However, the amount of ethylene converted by ACC and ethephon is not controllable, leaving it questionable whether either treatment can mimic the effects of ethylene for experiments that are sensitive to ethylene concentration, response window, and treatment durations. Ethylene can be chemically made by ethanol dehydration; however, further purification from the dehydration products is needed. We previously reported that the ethylene gas can be easily prepared by decomposing ethephon in a buffered condition and the resulting ethylene can be used directly. Ethylene responses can be estimated by the measurement of the hypocotyl length of etiolated seedlings, or byERF1(Ethylene Response Factor1) expression. Although ACC of low concentrations is insufficient to induceERF1expression, ACC of high concentrations can replace ethylene for experiments where ethylene treatment is not feasible. However, ACC may undergo early consumption. Versatile approaches were developed so that laboratories lacking ethylene and techniques for gas handling can easily perform necessary ethylene treatments.
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影响因子：
7.4
作者：
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通讯作者：
K. Woeste;Chen Ye;J. Kieber
影响因子：
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作者：
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影响因子：
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通讯作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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